Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice

Here, we present a protocol to create an in vivo lineage-tracing mouse model for mouse-papillomavirus-type 1 (MmuPV1)-infected cells. We describe the steps to generate and deliver the MmuPV1 lox-Cre-lox plasmid for the infection of mice, followed by skin tissue extraction and processing. We then det...

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Detalles Bibliográficos
Autores principales: Yilmaz, Vural, Strati, Katerina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826866/
https://www.ncbi.nlm.nih.gov/pubmed/36607810
http://dx.doi.org/10.1016/j.xpro.2022.101994
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author Yilmaz, Vural
Strati, Katerina
author_facet Yilmaz, Vural
Strati, Katerina
author_sort Yilmaz, Vural
collection PubMed
description Here, we present a protocol to create an in vivo lineage-tracing mouse model for mouse-papillomavirus-type 1 (MmuPV1)-infected cells. We describe the steps to generate and deliver the MmuPV1 lox-Cre-lox plasmid for the infection of mice, followed by skin tissue extraction and processing. We then detail how to use flow cytometry to trace, quantify, and analyze MmuPV1-harboring cells and their progeny. This model is suitable to investigate the early effects of papillomavirus on the target cells. For complete details on the use and execution of this protocol, please refer to Yilmaz et al. (2022).(1)
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spelling pubmed-98268662023-01-10 Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice Yilmaz, Vural Strati, Katerina STAR Protoc Protocol Here, we present a protocol to create an in vivo lineage-tracing mouse model for mouse-papillomavirus-type 1 (MmuPV1)-infected cells. We describe the steps to generate and deliver the MmuPV1 lox-Cre-lox plasmid for the infection of mice, followed by skin tissue extraction and processing. We then detail how to use flow cytometry to trace, quantify, and analyze MmuPV1-harboring cells and their progeny. This model is suitable to investigate the early effects of papillomavirus on the target cells. For complete details on the use and execution of this protocol, please refer to Yilmaz et al. (2022).(1) Elsevier 2023-01-04 /pmc/articles/PMC9826866/ /pubmed/36607810 http://dx.doi.org/10.1016/j.xpro.2022.101994 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yilmaz, Vural
Strati, Katerina
Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice
title Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice
title_full Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice
title_fullStr Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice
title_full_unstemmed Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice
title_short Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice
title_sort protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826866/
https://www.ncbi.nlm.nih.gov/pubmed/36607810
http://dx.doi.org/10.1016/j.xpro.2022.101994
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