An assay to evaluate the capacity of cholesterol acceptors using BODIPY-cholesterol in cells

Cholesterol is a structural component of cell membranes. Most cells are incapable of its catabolism, and intracellular cholesterol accumulation is linked to several disorders including cardiovascular and neurodegenerative diseases. Cholesterol efflux, essential to its metabolism, is dependent on acc...

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Detalles Bibliográficos
Autores principales: Raulin, Ana-Caroline, Liu, Chia-Chen, Bu, Guojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826879/
https://www.ncbi.nlm.nih.gov/pubmed/36598853
http://dx.doi.org/10.1016/j.xpro.2022.101976
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author Raulin, Ana-Caroline
Liu, Chia-Chen
Bu, Guojun
author_facet Raulin, Ana-Caroline
Liu, Chia-Chen
Bu, Guojun
author_sort Raulin, Ana-Caroline
collection PubMed
description Cholesterol is a structural component of cell membranes. Most cells are incapable of its catabolism, and intracellular cholesterol accumulation is linked to several disorders including cardiovascular and neurodegenerative diseases. Cholesterol efflux, essential to its metabolism, is dependent on acceptors such as apolipoproteins. Here, we describe an assay to evaluate the capacity of cholesterol acceptors. Cells are treated with an analog of cholesterol tagged with fluorescent BODIPY. Addition of an acceptor leads to BODIPY-cholesterol efflux, measured using a plate reader. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).(1)
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spelling pubmed-98268792023-01-10 An assay to evaluate the capacity of cholesterol acceptors using BODIPY-cholesterol in cells Raulin, Ana-Caroline Liu, Chia-Chen Bu, Guojun STAR Protoc Protocol Cholesterol is a structural component of cell membranes. Most cells are incapable of its catabolism, and intracellular cholesterol accumulation is linked to several disorders including cardiovascular and neurodegenerative diseases. Cholesterol efflux, essential to its metabolism, is dependent on acceptors such as apolipoproteins. Here, we describe an assay to evaluate the capacity of cholesterol acceptors. Cells are treated with an analog of cholesterol tagged with fluorescent BODIPY. Addition of an acceptor leads to BODIPY-cholesterol efflux, measured using a plate reader. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).(1) Elsevier 2023-01-03 /pmc/articles/PMC9826879/ /pubmed/36598853 http://dx.doi.org/10.1016/j.xpro.2022.101976 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Raulin, Ana-Caroline
Liu, Chia-Chen
Bu, Guojun
An assay to evaluate the capacity of cholesterol acceptors using BODIPY-cholesterol in cells
title An assay to evaluate the capacity of cholesterol acceptors using BODIPY-cholesterol in cells
title_full An assay to evaluate the capacity of cholesterol acceptors using BODIPY-cholesterol in cells
title_fullStr An assay to evaluate the capacity of cholesterol acceptors using BODIPY-cholesterol in cells
title_full_unstemmed An assay to evaluate the capacity of cholesterol acceptors using BODIPY-cholesterol in cells
title_short An assay to evaluate the capacity of cholesterol acceptors using BODIPY-cholesterol in cells
title_sort assay to evaluate the capacity of cholesterol acceptors using bodipy-cholesterol in cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826879/
https://www.ncbi.nlm.nih.gov/pubmed/36598853
http://dx.doi.org/10.1016/j.xpro.2022.101976
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