Cargando…

Liquid chromatography-mass spectrometry-based protocol to measure drug accumulation in Mycobacterium tuberculosis and its host cell

The extent to which a drug accumulates in Mycobacterium tuberculosis (Mtb) and its host cell can affect treatment efficacy. We describe protocols measuring drug accumulation in Mtb, macrophages, and Mtb-infected macrophages. The method leverages drug extraction from the cellular lysate and drug-leve...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ahn, Yong-Mo, Lavin, Richard C., Tan, Shumin, Freundlich, Joel S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826881/ https://www.ncbi.nlm.nih.gov/pubmed/36598855 http://dx.doi.org/10.1016/j.xpro.2022.101971

Ejemplares similares

Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry
por: Ogawa, Akiko, et al.
Publicado: (2021)

Protocol for analysis of liquid chromatography-mass spectrometry metabolomics data using R to understand how metabolites affect disease
por: Lin, Hong, et al.
Publicado: (2023)

Multiplexed targeted analysis of polyunsaturated fatty acids and oxylipins using liquid chromatography-tandem mass spectrometry
por: Turtoi, Evgenia, et al.
Publicado: (2023)

A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue
por: Dai, Di, et al.
Publicado: (2023)

Protocol to characterize target components of Gelsemium using an in-house herb database and high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry
por: Zuo, Meng-Ting, et al.
Publicado: (2023)

Back to the Basics of Liquid Chromatography-Mass Spectrometry
por: Kim, Young Jin, et al.
Publicado: (2022)

Quantitative Profiling of Bile Acids in Feces of Humans and Rodents by Ultra-High-Performance Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry
por: Zhang, Xiaoxu, et al.
Publicado: (2022)

Detection of Mycobacterium tuberculosis in Sputum by Gas Chromatography-Mass Spectrometry of Methyl Mycocerosates Released by Thermochemolysis
por: O'Sullivan, Denise M., et al.
Publicado: (2012)

Quantification of Protein Phosphorylation by Liquid Chromatography−Mass Spectrometry
por: Previs, Michael J., et al.
Publicado: (2008)

Ocrelizumab quantitation by liquid chromatography-tandem mass spectrometry
por: Hallin, Erik I., et al.
Publicado: (2022)

Liquid chromatography–tandem mass spectrometry for clinical diagnostics
por: Thomas, Stefani N., et al.
Publicado: (2022)

Targeting Mycobacterium tuberculosis response to environmental cues for the development of effective antitubercular drugs
por: Lavin, Richard C., et al.
Publicado: (2021)

Identification of Pyrrolizidine Alkaloids in Senecio Plants by Liquid Chromatography-Mass Spectrometry
por: Lu, An-Jing, et al.
Publicado: (2021)

Detection and Structural Characterization of Nucleophiles Trapped Reactive Metabolites of Limonin Using Liquid Chromatography-Mass Spectrometry
por: Deng, Yujie, et al.
Publicado: (2018)

Determination of ceftriaxone in human plasma using liquid chromatography–tandem mass spectrometry
por: Wongchang, Thamrong, et al.
Publicado: (2022)

Characterization of volatile thiols in Chinese liquor (Baijiu) by ultraperformance liquid chromatography–mass spectrometry and ultraperformance liquid chromatography–quadrupole-time-of-flight mass spectrometry
por: Yan, Yan, et al.
Publicado: (2022)

Determination of 25 quaternary ammonium compounds in sludge by liquid chromatography–mass spectrometry
por: Shu, Mushui, et al.
Publicado: (2023)

Quality analysis of Polygala tenuifolia root by ultrahigh performance liquid chromatography–tandem mass spectrometry and gas chromatography–mass spectrometry
por: Wu, Dan, et al.
Publicado: (2014)

Spatial relationships of intra-lesion heterogeneity in Mycobacterium tuberculosis microenvironment, replication status, and drug efficacy
por: Lavin, Richard C., et al.
Publicado: (2022)

Review of Peak Detection Algorithms in Liquid-Chromatography-Mass Spectrometry
por: Zhang, Jianqiu, et al.
Publicado: (2009)

Noncovalent Dimer Formation in Liquid Chromatography–Mass Spectrometry Analysis
por: Medzihradszky, Katalin F.
Publicado: (2014)

Liquid Chromatography–mass Spectrometry Analysis of Carvacrol in Chicken Tissues
por: Śniegocki, Tomasz
Publicado: (2022)

Liquid Chromatography–Tandem Mass Spectrometry in Newborn Screening Laboratories
por: Gelb, Michael H., et al.
Publicado: (2022)

Serum Metabolomic Profiling in Aging Mice Using Liquid Chromatography—Mass Spectrometry
por: Yue, Tong, et al.
Publicado: (2022)

Simultaneous Method for Selected PBDEs and HBCDDs in Foodstuffs Using Gas Chromatography—Tandem Mass Spectrometry and Liquid Chromatography—Tandem Mass Spectrometry
por: Lipičar, Eva, et al.
Publicado: (2022)

Liquid Chromatography–Mass Spectrometry/Mass Spectrometry Analysis and Pharmacokinetic Assessment of Ponatinib in Sprague–Dawley Rats
por: Wang, Pei, et al.
Publicado: (2016)

Correlating Mass Spectrometry Imaging and Liquid Chromatography-Tandem Mass Spectrometry for Tissue-Based Pharmacokinetic Studies
por: Dannhorn, Andreas, et al.
Publicado: (2022)

Quantitation of paracetamol by liquid chromatography–mass spectrometry in human plasma in support of clinical trial
por: Kam, Richard Kin-Ting, et al.
Publicado: (2018)

Identification and Quantification of Dimethylamylamine in Geranium by Liquid Chromatography Tandem Mass Spectrometry
por: Li, J.S., et al.
Publicado: (2012)

Measurement of tamsulosin in human serum by liquid chromatography–tandem mass spectrometry()
por: Upreti, Rita, et al.
Publicado: (2013)

Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry
por: Asgari, Arezoo, et al.
Publicado: (2015)

Combined Liquid Chromatography–Tandem Mass Spectrometry Analysis of Progesterone Metabolites
por: Sinreih, Maša, et al.
Publicado: (2015)

Clinical Glycomics Employing Graphitized Carbon Liquid Chromatography–Mass Spectrometry
por: Stavenhagen, Kathrin, et al.
Publicado: (2014)

Bayesian ABC-MCMC Classification of Liquid Chromatography–Mass Spectrometry Data
por: Banerjee, Upamanyu, et al.
Publicado: (2017)

Pyrrolizidine Alkaloids in Honey: Determination with Liquid Chromatography-mass Spectrometry Method
por: Kowalczyk, Ewelina, et al.
Publicado: (2018)

Comprehensive metabolic profiling of Parkinson’s disease by liquid chromatography-mass spectrometry
por: Shao, Yaping, et al.
Publicado: (2021)

Analysis of Bacteriohopanoids from Thermophilic Bacteria by Liquid Chromatography–Mass Spectrometry
por: Kolouchová, Irena, et al.
Publicado: (2021)

Detection of Porphyrins in Hair Using Capillary Liquid Chromatography-Mass Spectrometry
por: Louleb, Marwa, et al.
Publicado: (2022)

Use, history, and liquid chromatography/mass spectrometry chemical analysis of Aconitum
por: El-Shazly, Mohamed, et al.
Publicado: (2015)

Establishment to measure oxycodone in plasma with liquid chromatography–tandem mass spectrometry
por: Ito, Suguru, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_b576lnevl8e4ka2fq8b15un461