Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise

The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mic...

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Detalles Bibliográficos
Autores principales: Ashcroft, Stephen P., Jepsen, Sara L., Ehrlich, Amy M., Treebak, Jonas T., Holst, Jens J., Zierath, Juleen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826965/
https://www.ncbi.nlm.nih.gov/pubmed/36602899
http://dx.doi.org/10.1016/j.xpro.2022.101985
Descripción
Sumario:The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mice and the region-specific sampling of blood from the liver and hindlimb. This procedure is particularly relevant for the study of tissue-specific metabolism in response to exercise. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022).(1)

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