Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise

The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mic...

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Autores principales: Ashcroft, Stephen P., Jepsen, Sara L., Ehrlich, Amy M., Treebak, Jonas T., Holst, Jens J., Zierath, Juleen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826965/
https://www.ncbi.nlm.nih.gov/pubmed/36602899
http://dx.doi.org/10.1016/j.xpro.2022.101985
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author Ashcroft, Stephen P.
Jepsen, Sara L.
Ehrlich, Amy M.
Treebak, Jonas T.
Holst, Jens J.
Zierath, Juleen R.
author_facet Ashcroft, Stephen P.
Jepsen, Sara L.
Ehrlich, Amy M.
Treebak, Jonas T.
Holst, Jens J.
Zierath, Juleen R.
author_sort Ashcroft, Stephen P.
collection PubMed
description The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mice and the region-specific sampling of blood from the liver and hindlimb. This procedure is particularly relevant for the study of tissue-specific metabolism in response to exercise. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022).(1)
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spelling pubmed-98269652023-01-10 Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise Ashcroft, Stephen P. Jepsen, Sara L. Ehrlich, Amy M. Treebak, Jonas T. Holst, Jens J. Zierath, Juleen R. STAR Protoc Protocol The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mice and the region-specific sampling of blood from the liver and hindlimb. This procedure is particularly relevant for the study of tissue-specific metabolism in response to exercise. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022).(1) Elsevier 2023-01-03 /pmc/articles/PMC9826965/ /pubmed/36602899 http://dx.doi.org/10.1016/j.xpro.2022.101985 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Ashcroft, Stephen P.
Jepsen, Sara L.
Ehrlich, Amy M.
Treebak, Jonas T.
Holst, Jens J.
Zierath, Juleen R.
Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise
title Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise
title_full Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise
title_fullStr Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise
title_full_unstemmed Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise
title_short Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise
title_sort protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826965/
https://www.ncbi.nlm.nih.gov/pubmed/36602899
http://dx.doi.org/10.1016/j.xpro.2022.101985
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