Protocol for investigating the effect of food digestion in C. elegans on development by feeding the inedible bacteria Staphylococcus saprophyticus

The digestive system works by moving food through the gastrointestinal tract, which processes food into molecules that can be absorbed and utilized by the cells of the body. However, little is known about the signaling pathways that regulate food digestion. Here, by using the inedible bacteria (Staphylococcus saprophyticus)-worm culture system, we established a simple food digestion methodology, which is of low cost and high efficiency, to explore molecular mechanisms underlying food digestion in animals. For complete details on the use and execution of this protocol, please refer to Geng et al. (2022).(1)