Cargando…
Prevalent and immunodominant CD8 T cell epitopes are conserved in SARS-CoV-2 variants
The emergence of SARS-CoV-2 variants of concern (VOC) is driven by mutations that mediate escape from neutralizing antibodies. There is also evidence that mutations can cause loss of T cell epitopes. However, studies on viral escape from T cell immunity have been hampered by uncertain estimates of e...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Authors.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826989/ https://www.ncbi.nlm.nih.gov/pubmed/36656713 http://dx.doi.org/10.1016/j.celrep.2023.111995 |
_version_ | 1784866980848730112 |
---|---|
author | Meyer, Saskia Blaas, Isaac Bollineni, Ravi Chand Delic-Sarac, Marina Tran, Trung T. Knetter, Cathrine Dai, Ke-Zheng Madssen, Torfinn Støve Vaage, John T. Gustavsen, Alice Yang, Weiwen Nissen-Meyer, Lise Sofie Haug Douvlataniotis, Karolos Laos, Maarja Nielsen, Morten Milek Thiede, Bernd Søraas, Arne Lund-Johansen, Fridtjof Rustad, Even H. Olweus, Johanna |
author_facet | Meyer, Saskia Blaas, Isaac Bollineni, Ravi Chand Delic-Sarac, Marina Tran, Trung T. Knetter, Cathrine Dai, Ke-Zheng Madssen, Torfinn Støve Vaage, John T. Gustavsen, Alice Yang, Weiwen Nissen-Meyer, Lise Sofie Haug Douvlataniotis, Karolos Laos, Maarja Nielsen, Morten Milek Thiede, Bernd Søraas, Arne Lund-Johansen, Fridtjof Rustad, Even H. Olweus, Johanna |
author_sort | Meyer, Saskia |
collection | PubMed |
description | The emergence of SARS-CoV-2 variants of concern (VOC) is driven by mutations that mediate escape from neutralizing antibodies. There is also evidence that mutations can cause loss of T cell epitopes. However, studies on viral escape from T cell immunity have been hampered by uncertain estimates of epitope prevalence. Here, we map and quantify CD8 T cell responses to SARS-CoV-2-specific minimal epitopes in blood drawn from April to June 2020 from 83 COVID-19 convalescents. Among 37 HLA ligands eluted from five prevalent alleles and an additional 86 predicted binders, we identify 29 epitopes with an immunoprevalence ranging from 3% to 100% among individuals expressing the relevant HLA allele. Mutations in VOC are reported in 10.3% of the epitopes, while 20.6% of the non-immunogenic peptides are mutated in VOC. The nine most prevalent epitopes are conserved in VOC. Thus, comprehensive mapping of epitope prevalence does not provide evidence that mutations in VOC are driven by escape of T cell immunity. |
format | Online Article Text |
id | pubmed-9826989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Authors. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98269892023-01-09 Prevalent and immunodominant CD8 T cell epitopes are conserved in SARS-CoV-2 variants Meyer, Saskia Blaas, Isaac Bollineni, Ravi Chand Delic-Sarac, Marina Tran, Trung T. Knetter, Cathrine Dai, Ke-Zheng Madssen, Torfinn Støve Vaage, John T. Gustavsen, Alice Yang, Weiwen Nissen-Meyer, Lise Sofie Haug Douvlataniotis, Karolos Laos, Maarja Nielsen, Morten Milek Thiede, Bernd Søraas, Arne Lund-Johansen, Fridtjof Rustad, Even H. Olweus, Johanna Cell Rep Article The emergence of SARS-CoV-2 variants of concern (VOC) is driven by mutations that mediate escape from neutralizing antibodies. There is also evidence that mutations can cause loss of T cell epitopes. However, studies on viral escape from T cell immunity have been hampered by uncertain estimates of epitope prevalence. Here, we map and quantify CD8 T cell responses to SARS-CoV-2-specific minimal epitopes in blood drawn from April to June 2020 from 83 COVID-19 convalescents. Among 37 HLA ligands eluted from five prevalent alleles and an additional 86 predicted binders, we identify 29 epitopes with an immunoprevalence ranging from 3% to 100% among individuals expressing the relevant HLA allele. Mutations in VOC are reported in 10.3% of the epitopes, while 20.6% of the non-immunogenic peptides are mutated in VOC. The nine most prevalent epitopes are conserved in VOC. Thus, comprehensive mapping of epitope prevalence does not provide evidence that mutations in VOC are driven by escape of T cell immunity. The Authors. 2023-01-31 2023-01-09 /pmc/articles/PMC9826989/ /pubmed/36656713 http://dx.doi.org/10.1016/j.celrep.2023.111995 Text en © 2023 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Meyer, Saskia Blaas, Isaac Bollineni, Ravi Chand Delic-Sarac, Marina Tran, Trung T. Knetter, Cathrine Dai, Ke-Zheng Madssen, Torfinn Støve Vaage, John T. Gustavsen, Alice Yang, Weiwen Nissen-Meyer, Lise Sofie Haug Douvlataniotis, Karolos Laos, Maarja Nielsen, Morten Milek Thiede, Bernd Søraas, Arne Lund-Johansen, Fridtjof Rustad, Even H. Olweus, Johanna Prevalent and immunodominant CD8 T cell epitopes are conserved in SARS-CoV-2 variants |
title | Prevalent and immunodominant CD8 T cell epitopes are conserved in SARS-CoV-2 variants |
title_full | Prevalent and immunodominant CD8 T cell epitopes are conserved in SARS-CoV-2 variants |
title_fullStr | Prevalent and immunodominant CD8 T cell epitopes are conserved in SARS-CoV-2 variants |
title_full_unstemmed | Prevalent and immunodominant CD8 T cell epitopes are conserved in SARS-CoV-2 variants |
title_short | Prevalent and immunodominant CD8 T cell epitopes are conserved in SARS-CoV-2 variants |
title_sort | prevalent and immunodominant cd8 t cell epitopes are conserved in sars-cov-2 variants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826989/ https://www.ncbi.nlm.nih.gov/pubmed/36656713 http://dx.doi.org/10.1016/j.celrep.2023.111995 |
work_keys_str_mv | AT meyersaskia prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT blaasisaac prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT bollineniravichand prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT delicsaracmarina prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT trantrungt prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT knettercathrine prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT daikezheng prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT madssentorfinnstøve prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT vaagejohnt prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT gustavsenalice prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT yangweiwen prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT nissenmeyerlisesofiehaug prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT douvlataniotiskarolos prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT laosmaarja prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT nielsenmortenmilek prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT thiedebernd prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT søraasarne prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT lundjohansenfridtjof prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT rustadevenh prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants AT olweusjohanna prevalentandimmunodominantcd8tcellepitopesareconservedinsarscov2variants |