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ATAC-seq and RNA-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken

BACKGROUND: Sex determination and differentiation are complex and delicate processes. In female chickens, the process of sex differentiation is sensitive and prone to be affected by the administration of aromatase inhibitors, which result in chicken sex reversal and infertility. However, the molecul...

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Autores principales: Zhang, Xiuan, Li, Jianbo, Wang, Xiqiong, Jie, Yuchen, Sun, Congjiao, Zheng, Jiangxia, Li, Junying, Yang, Ning, Chen, Sirui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9827654/
https://www.ncbi.nlm.nih.gov/pubmed/36617567
http://dx.doi.org/10.1186/s13072-022-00476-1
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author Zhang, Xiuan
Li, Jianbo
Wang, Xiqiong
Jie, Yuchen
Sun, Congjiao
Zheng, Jiangxia
Li, Junying
Yang, Ning
Chen, Sirui
author_facet Zhang, Xiuan
Li, Jianbo
Wang, Xiqiong
Jie, Yuchen
Sun, Congjiao
Zheng, Jiangxia
Li, Junying
Yang, Ning
Chen, Sirui
author_sort Zhang, Xiuan
collection PubMed
description BACKGROUND: Sex determination and differentiation are complex and delicate processes. In female chickens, the process of sex differentiation is sensitive and prone to be affected by the administration of aromatase inhibitors, which result in chicken sex reversal and infertility. However, the molecular mechanisms underlying sex differentiation and infertility in chicken sex reversal remain unclear. Therefore, we established a sex-reversed chicken flock by injecting an aromatase inhibitor, fadrozole, and constructed relatively high-resolution profiles of the gene expression and chromatin accessibility of embryonic gonads. RESULTS: We revealed that fadrozole affected the transcriptional activities of several genes, such as DMRT1, SOX9, FOXL2, and CYP19A1, related to sex determination and differentiation, and the expression of a set of gonadal development-related genes, such as FGFR3 and TOX3, by regulating nearby open chromatin regions in sex-reversed chicken embryos. After sexual maturity, the sex-reversed chickens were confirmed to be infertile, and the possible causes of this infertility were further investigated. We found that the structure of the gonads and sperm were greatly deformed, and we identified several promising genes related to spermatogenesis and infertility, such as SPEF2, DNAI1, and TACR3, through RNA-seq. CONCLUSIONS: This study provides clear insights into the exploration of potential molecular basis underlying sex differentiation and infertility in sex-reversed chickens and lays a foundation for further research into the sex development of birds. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13072-022-00476-1.
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spelling pubmed-98276542023-01-10 ATAC-seq and RNA-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken Zhang, Xiuan Li, Jianbo Wang, Xiqiong Jie, Yuchen Sun, Congjiao Zheng, Jiangxia Li, Junying Yang, Ning Chen, Sirui Epigenetics Chromatin Research BACKGROUND: Sex determination and differentiation are complex and delicate processes. In female chickens, the process of sex differentiation is sensitive and prone to be affected by the administration of aromatase inhibitors, which result in chicken sex reversal and infertility. However, the molecular mechanisms underlying sex differentiation and infertility in chicken sex reversal remain unclear. Therefore, we established a sex-reversed chicken flock by injecting an aromatase inhibitor, fadrozole, and constructed relatively high-resolution profiles of the gene expression and chromatin accessibility of embryonic gonads. RESULTS: We revealed that fadrozole affected the transcriptional activities of several genes, such as DMRT1, SOX9, FOXL2, and CYP19A1, related to sex determination and differentiation, and the expression of a set of gonadal development-related genes, such as FGFR3 and TOX3, by regulating nearby open chromatin regions in sex-reversed chicken embryos. After sexual maturity, the sex-reversed chickens were confirmed to be infertile, and the possible causes of this infertility were further investigated. We found that the structure of the gonads and sperm were greatly deformed, and we identified several promising genes related to spermatogenesis and infertility, such as SPEF2, DNAI1, and TACR3, through RNA-seq. CONCLUSIONS: This study provides clear insights into the exploration of potential molecular basis underlying sex differentiation and infertility in sex-reversed chickens and lays a foundation for further research into the sex development of birds. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13072-022-00476-1. BioMed Central 2023-01-09 /pmc/articles/PMC9827654/ /pubmed/36617567 http://dx.doi.org/10.1186/s13072-022-00476-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Xiuan
Li, Jianbo
Wang, Xiqiong
Jie, Yuchen
Sun, Congjiao
Zheng, Jiangxia
Li, Junying
Yang, Ning
Chen, Sirui
ATAC-seq and RNA-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken
title ATAC-seq and RNA-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken
title_full ATAC-seq and RNA-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken
title_fullStr ATAC-seq and RNA-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken
title_full_unstemmed ATAC-seq and RNA-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken
title_short ATAC-seq and RNA-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken
title_sort atac-seq and rna-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9827654/
https://www.ncbi.nlm.nih.gov/pubmed/36617567
http://dx.doi.org/10.1186/s13072-022-00476-1
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