Cargando…

A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa

The control of cellular zinc (Zn) concentrations by dedicated import and export systems is essential for the survival and virulence of Pseudomonas aeruginosa. The transcription of its many Zn transporters is therefore tightly regulated by a known set of transcription factors involved in either the i...

Descripción completa

Detalles Bibliográficos
Autores principales: Ducret, Verena, Gonzalez, Diego, Leoni, Sara, Valentini, Martina, Perron, Karl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9827704/
https://www.ncbi.nlm.nih.gov/pubmed/36617571
http://dx.doi.org/10.1186/s12866-022-02750-4
_version_ 1784867104452771840
author Ducret, Verena
Gonzalez, Diego
Leoni, Sara
Valentini, Martina
Perron, Karl
author_facet Ducret, Verena
Gonzalez, Diego
Leoni, Sara
Valentini, Martina
Perron, Karl
author_sort Ducret, Verena
collection PubMed
description The control of cellular zinc (Zn) concentrations by dedicated import and export systems is essential for the survival and virulence of Pseudomonas aeruginosa. The transcription of its many Zn transporters is therefore tightly regulated by a known set of transcription factors involved in either the import or the export of Zn. In this work, we show that the Zur protein, a well-known repressor of Zn import, plays a dual role and functions in both import and export processes. In a situation of Zn excess, Zur represses Zn entry, but also activates the transcription of czcR, a positive regulator of the Zn export system. To achieve this, Zur binds at two sites, located by DNA footprinting in the region downstream the czcR transcription start site. In agreement with this regulation, a delay in induction of the efflux system is observed in the absence of Zur and Zn resistance is reduced. The discovery of this regulation highlights a new role of Zur as global regulator of Zn homeostasis in P. aeruginosa disclosing an important link between Zur and zinc export. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02750-4.
format Online
Article
Text
id pubmed-9827704
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-98277042023-01-10 A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa Ducret, Verena Gonzalez, Diego Leoni, Sara Valentini, Martina Perron, Karl BMC Microbiol Research The control of cellular zinc (Zn) concentrations by dedicated import and export systems is essential for the survival and virulence of Pseudomonas aeruginosa. The transcription of its many Zn transporters is therefore tightly regulated by a known set of transcription factors involved in either the import or the export of Zn. In this work, we show that the Zur protein, a well-known repressor of Zn import, plays a dual role and functions in both import and export processes. In a situation of Zn excess, Zur represses Zn entry, but also activates the transcription of czcR, a positive regulator of the Zn export system. To achieve this, Zur binds at two sites, located by DNA footprinting in the region downstream the czcR transcription start site. In agreement with this regulation, a delay in induction of the efflux system is observed in the absence of Zur and Zn resistance is reduced. The discovery of this regulation highlights a new role of Zur as global regulator of Zn homeostasis in P. aeruginosa disclosing an important link between Zur and zinc export. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02750-4. BioMed Central 2023-01-09 /pmc/articles/PMC9827704/ /pubmed/36617571 http://dx.doi.org/10.1186/s12866-022-02750-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ducret, Verena
Gonzalez, Diego
Leoni, Sara
Valentini, Martina
Perron, Karl
A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa
title A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa
title_full A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa
title_fullStr A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa
title_full_unstemmed A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa
title_short A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa
title_sort zur-mediated transcriptional regulation of the zinc export system in pseudomonas aeruginosa
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9827704/
https://www.ncbi.nlm.nih.gov/pubmed/36617571
http://dx.doi.org/10.1186/s12866-022-02750-4
work_keys_str_mv AT ducretverena azurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa
AT gonzalezdiego azurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa
AT leonisara azurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa
AT valentinimartina azurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa
AT perronkarl azurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa
AT ducretverena zurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa
AT gonzalezdiego zurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa
AT leonisara zurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa
AT valentinimartina zurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa
AT perronkarl zurmediatedtranscriptionalregulationofthezincexportsysteminpseudomonasaeruginosa