Selection and validation of reference genes for qPCR analysis of differentiation and maturation of THP‐1 cells into M1 macrophage‐like cells

For cell‐based assays studying monocytes and macrophages, the immortalized monocyte cell line THP‐1 is widely used and stimulated with phorbol 12‐myristate 13‐acetate, lipopolysaccharide (LPS) and/or interferon‐γ (IFN‐γ), after which it differentiates and polarizes into proinflammatory M1‐like macro...

Descripción completa

Detalles Bibliográficos
Autores principales: Ren, Guoqiang, Juhl, Morten, Peng, Qiuyue, Fink, Trine, Porsborg, Simone Riis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828170/
https://www.ncbi.nlm.nih.gov/pubmed/36184577
http://dx.doi.org/10.1111/imcb.12590

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828170/
https://www.ncbi.nlm.nih.gov/pubmed/36184577
http://dx.doi.org/10.1111/imcb.12590

Ejemplares similares