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Mechanistic Insights into the Phase Separation Behavior and Pathway‐Directed Information Exchange in all‐DNA Droplets
Liquid‐liquid phase separation provides a versatile approach to fabricating cell‐mimicking coacervates. Recently, it was discovered that phase separation of single‐stranded DNA (ssDNA) allows for forming protocells and microgels in multicomponent systems. However, the mechanism of the ssDNA phase se...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828218/ https://www.ncbi.nlm.nih.gov/pubmed/36112754 http://dx.doi.org/10.1002/anie.202208951 |
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author | Liu, Wei Samanta, Avik Deng, Jie Akintayo, Cecilia Oluwadunsin Walther, Andreas |
author_facet | Liu, Wei Samanta, Avik Deng, Jie Akintayo, Cecilia Oluwadunsin Walther, Andreas |
author_sort | Liu, Wei |
collection | PubMed |
description | Liquid‐liquid phase separation provides a versatile approach to fabricating cell‐mimicking coacervates. Recently, it was discovered that phase separation of single‐stranded DNA (ssDNA) allows for forming protocells and microgels in multicomponent systems. However, the mechanism of the ssDNA phase separation is not comprehensively understood. Here, we present mechanistic insights into the metal‐dependent phase separation of ssDNA and leverage this understanding for a straightforward formation of all‐DNA droplets. Two phase separation temperatures are found that correspond to the formation of primary nuclei and a growth process. Ca(2+) allows for irreversible, whereas Mg(2+) leads to reversible phase separation. Capitalizing on these differences makes it possible to control the information transfer of one‐component DNA droplets and two‐component core–shell protocells. This study introduces new kinetic traps of phase separating ssDNA that lead to new phenomena in cell‐mimicking systems. |
format | Online Article Text |
id | pubmed-9828218 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98282182023-01-10 Mechanistic Insights into the Phase Separation Behavior and Pathway‐Directed Information Exchange in all‐DNA Droplets Liu, Wei Samanta, Avik Deng, Jie Akintayo, Cecilia Oluwadunsin Walther, Andreas Angew Chem Int Ed Engl Research Articles Liquid‐liquid phase separation provides a versatile approach to fabricating cell‐mimicking coacervates. Recently, it was discovered that phase separation of single‐stranded DNA (ssDNA) allows for forming protocells and microgels in multicomponent systems. However, the mechanism of the ssDNA phase separation is not comprehensively understood. Here, we present mechanistic insights into the metal‐dependent phase separation of ssDNA and leverage this understanding for a straightforward formation of all‐DNA droplets. Two phase separation temperatures are found that correspond to the formation of primary nuclei and a growth process. Ca(2+) allows for irreversible, whereas Mg(2+) leads to reversible phase separation. Capitalizing on these differences makes it possible to control the information transfer of one‐component DNA droplets and two‐component core–shell protocells. This study introduces new kinetic traps of phase separating ssDNA that lead to new phenomena in cell‐mimicking systems. John Wiley and Sons Inc. 2022-10-07 2022-11-07 /pmc/articles/PMC9828218/ /pubmed/36112754 http://dx.doi.org/10.1002/anie.202208951 Text en © 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Research Articles Liu, Wei Samanta, Avik Deng, Jie Akintayo, Cecilia Oluwadunsin Walther, Andreas Mechanistic Insights into the Phase Separation Behavior and Pathway‐Directed Information Exchange in all‐DNA Droplets |
title | Mechanistic Insights into the Phase Separation Behavior and Pathway‐Directed Information Exchange in all‐DNA Droplets |
title_full | Mechanistic Insights into the Phase Separation Behavior and Pathway‐Directed Information Exchange in all‐DNA Droplets |
title_fullStr | Mechanistic Insights into the Phase Separation Behavior and Pathway‐Directed Information Exchange in all‐DNA Droplets |
title_full_unstemmed | Mechanistic Insights into the Phase Separation Behavior and Pathway‐Directed Information Exchange in all‐DNA Droplets |
title_short | Mechanistic Insights into the Phase Separation Behavior and Pathway‐Directed Information Exchange in all‐DNA Droplets |
title_sort | mechanistic insights into the phase separation behavior and pathway‐directed information exchange in all‐dna droplets |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828218/ https://www.ncbi.nlm.nih.gov/pubmed/36112754 http://dx.doi.org/10.1002/anie.202208951 |
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