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miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells

The occurrence of acute rejection after liver transplantation seriously impairs the prognosis of patients. miRNA is involved in many physiological and pathological processes of the body, but the mechanism of miRNA action in liver transplantation is not completely clear. In this study, we discuss the...

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Autores principales: Chai, Hao, Lei, Zilun, Liu, Yanyao, Gong, Junhua, Cao, Zhenrui, Huang, Zuotian, Yang, Hang, Wu, Zhongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828294/
https://www.ncbi.nlm.nih.gov/pubmed/35959879
http://dx.doi.org/10.3724/abbs.2022100
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author Chai, Hao
Lei, Zilun
Liu, Yanyao
Gong, Junhua
Cao, Zhenrui
Huang, Zuotian
Yang, Hang
Wu, Zhongjun
author_facet Chai, Hao
Lei, Zilun
Liu, Yanyao
Gong, Junhua
Cao, Zhenrui
Huang, Zuotian
Yang, Hang
Wu, Zhongjun
author_sort Chai, Hao
collection PubMed
description The occurrence of acute rejection after liver transplantation seriously impairs the prognosis of patients. miRNA is involved in many physiological and pathological processes of the body, but the mechanism of miRNA action in liver transplantation is not completely clear. In this study, we discuss the role of miR-505-5p in acute rejection after liver transplantation and its putative regulating mechanism. We construct an allogeneic rat liver transplantation model, observe the morphological and pathological changes in liver tissue, detect the expression levels of Myd88, miR-505-5p, IL-10 and TNF-α, and confirm that Myd88 is one of the direct targets of miR-505. The effects of miR-505-5p on the Myd88/TRAF6/NF-κB and MAPK pathways are detected both in vitro and in vivo, and the standard markers of Kupffer cell M1/M2 polarization are also detected. The results of qRT-PCR experiments show that miR-505-5p has a downward trend in rats with acute rejection. Western blot analysis reveals that over-expression of miR-505-5p induces the reduction of NF-κB and MAPK pathways both in vitro and in vivo. The role of miR-505-5p in alleviating acute rejection after transplantation may be accomplished by inducing M2-type polarization of Kupffer cells. In conclusion, we find that miR-505-5p alleviates acute rejection of liver transplantation by inducing M2 polarization of macrophages via the Myd88/TRAF6 axis, which suggests a potential strategy based on miRNAs in the follow-up treatment of liver transplantation.
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spelling pubmed-98282942023-02-10 miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells Chai, Hao Lei, Zilun Liu, Yanyao Gong, Junhua Cao, Zhenrui Huang, Zuotian Yang, Hang Wu, Zhongjun Acta Biochim Biophys Sin (Shanghai) Research Article The occurrence of acute rejection after liver transplantation seriously impairs the prognosis of patients. miRNA is involved in many physiological and pathological processes of the body, but the mechanism of miRNA action in liver transplantation is not completely clear. In this study, we discuss the role of miR-505-5p in acute rejection after liver transplantation and its putative regulating mechanism. We construct an allogeneic rat liver transplantation model, observe the morphological and pathological changes in liver tissue, detect the expression levels of Myd88, miR-505-5p, IL-10 and TNF-α, and confirm that Myd88 is one of the direct targets of miR-505. The effects of miR-505-5p on the Myd88/TRAF6/NF-κB and MAPK pathways are detected both in vitro and in vivo, and the standard markers of Kupffer cell M1/M2 polarization are also detected. The results of qRT-PCR experiments show that miR-505-5p has a downward trend in rats with acute rejection. Western blot analysis reveals that over-expression of miR-505-5p induces the reduction of NF-κB and MAPK pathways both in vitro and in vivo. The role of miR-505-5p in alleviating acute rejection after transplantation may be accomplished by inducing M2-type polarization of Kupffer cells. In conclusion, we find that miR-505-5p alleviates acute rejection of liver transplantation by inducing M2 polarization of macrophages via the Myd88/TRAF6 axis, which suggests a potential strategy based on miRNAs in the follow-up treatment of liver transplantation. Oxford University Press 2022-08-08 /pmc/articles/PMC9828294/ /pubmed/35959879 http://dx.doi.org/10.3724/abbs.2022100 Text en © The Author(s) 2021. https://creativecommons.org/licenses/by-nc/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Research Article
Chai, Hao
Lei, Zilun
Liu, Yanyao
Gong, Junhua
Cao, Zhenrui
Huang, Zuotian
Yang, Hang
Wu, Zhongjun
miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells
title miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells
title_full miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells
title_fullStr miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells
title_full_unstemmed miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells
title_short miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells
title_sort mir-505-5p alleviates acute rejection of liver transplantation by inhibiting myd88 and inducing m2 polarizationof kupffer cells: mir-505-5p affects m2 polarization of kupffer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828294/
https://www.ncbi.nlm.nih.gov/pubmed/35959879
http://dx.doi.org/10.3724/abbs.2022100
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