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miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells
The occurrence of acute rejection after liver transplantation seriously impairs the prognosis of patients. miRNA is involved in many physiological and pathological processes of the body, but the mechanism of miRNA action in liver transplantation is not completely clear. In this study, we discuss the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828294/ https://www.ncbi.nlm.nih.gov/pubmed/35959879 http://dx.doi.org/10.3724/abbs.2022100 |
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author | Chai, Hao Lei, Zilun Liu, Yanyao Gong, Junhua Cao, Zhenrui Huang, Zuotian Yang, Hang Wu, Zhongjun |
author_facet | Chai, Hao Lei, Zilun Liu, Yanyao Gong, Junhua Cao, Zhenrui Huang, Zuotian Yang, Hang Wu, Zhongjun |
author_sort | Chai, Hao |
collection | PubMed |
description | The occurrence of acute rejection after liver transplantation seriously impairs the prognosis of patients. miRNA is involved in many physiological and pathological processes of the body, but the mechanism of miRNA action in liver transplantation is not completely clear. In this study, we discuss the role of miR-505-5p in acute rejection after liver transplantation and its putative regulating mechanism. We construct an allogeneic rat liver transplantation model, observe the morphological and pathological changes in liver tissue, detect the expression levels of Myd88, miR-505-5p, IL-10 and TNF-α, and confirm that Myd88 is one of the direct targets of miR-505. The effects of miR-505-5p on the Myd88/TRAF6/NF-κB and MAPK pathways are detected both in vitro and in vivo, and the standard markers of Kupffer cell M1/M2 polarization are also detected. The results of qRT-PCR experiments show that miR-505-5p has a downward trend in rats with acute rejection. Western blot analysis reveals that over-expression of miR-505-5p induces the reduction of NF-κB and MAPK pathways both in vitro and in vivo. The role of miR-505-5p in alleviating acute rejection after transplantation may be accomplished by inducing M2-type polarization of Kupffer cells. In conclusion, we find that miR-505-5p alleviates acute rejection of liver transplantation by inducing M2 polarization of macrophages via the Myd88/TRAF6 axis, which suggests a potential strategy based on miRNAs in the follow-up treatment of liver transplantation. |
format | Online Article Text |
id | pubmed-9828294 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-98282942023-02-10 miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells Chai, Hao Lei, Zilun Liu, Yanyao Gong, Junhua Cao, Zhenrui Huang, Zuotian Yang, Hang Wu, Zhongjun Acta Biochim Biophys Sin (Shanghai) Research Article The occurrence of acute rejection after liver transplantation seriously impairs the prognosis of patients. miRNA is involved in many physiological and pathological processes of the body, but the mechanism of miRNA action in liver transplantation is not completely clear. In this study, we discuss the role of miR-505-5p in acute rejection after liver transplantation and its putative regulating mechanism. We construct an allogeneic rat liver transplantation model, observe the morphological and pathological changes in liver tissue, detect the expression levels of Myd88, miR-505-5p, IL-10 and TNF-α, and confirm that Myd88 is one of the direct targets of miR-505. The effects of miR-505-5p on the Myd88/TRAF6/NF-κB and MAPK pathways are detected both in vitro and in vivo, and the standard markers of Kupffer cell M1/M2 polarization are also detected. The results of qRT-PCR experiments show that miR-505-5p has a downward trend in rats with acute rejection. Western blot analysis reveals that over-expression of miR-505-5p induces the reduction of NF-κB and MAPK pathways both in vitro and in vivo. The role of miR-505-5p in alleviating acute rejection after transplantation may be accomplished by inducing M2-type polarization of Kupffer cells. In conclusion, we find that miR-505-5p alleviates acute rejection of liver transplantation by inducing M2 polarization of macrophages via the Myd88/TRAF6 axis, which suggests a potential strategy based on miRNAs in the follow-up treatment of liver transplantation. Oxford University Press 2022-08-08 /pmc/articles/PMC9828294/ /pubmed/35959879 http://dx.doi.org/10.3724/abbs.2022100 Text en © The Author(s) 2021. https://creativecommons.org/licenses/by-nc/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/). |
spellingShingle | Research Article Chai, Hao Lei, Zilun Liu, Yanyao Gong, Junhua Cao, Zhenrui Huang, Zuotian Yang, Hang Wu, Zhongjun miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells |
title | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells |
title_full | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells |
title_fullStr | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells |
title_full_unstemmed | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells |
title_short | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells: miR-505-5p affects M2 polarization of Kupffer cells |
title_sort | mir-505-5p alleviates acute rejection of liver transplantation by inhibiting myd88 and inducing m2 polarizationof kupffer cells: mir-505-5p affects m2 polarization of kupffer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828294/ https://www.ncbi.nlm.nih.gov/pubmed/35959879 http://dx.doi.org/10.3724/abbs.2022100 |
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