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Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b (6) f

Photosynthesis is fundamental for plant growth and yield. The cytochrome b (6) f complex catalyses a rate‐limiting step in thylakoid electron transport and therefore represents an important point of regulation of photosynthesis. Here we show that overexpression of a single core subunit of cytochrome...

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Autores principales: Heyno, Eiri, Ermakova, Maria, Lopez‐Calcagno, Patricia E., Woodford, Russell, Brown, Kenny L., Matthews, Jack S. A., Osmond, Barry, Raines, Christine A., von Caemmerer, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828649/
https://www.ncbi.nlm.nih.gov/pubmed/36259085
http://dx.doi.org/10.1111/ppl.13803
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author Heyno, Eiri
Ermakova, Maria
Lopez‐Calcagno, Patricia E.
Woodford, Russell
Brown, Kenny L.
Matthews, Jack S. A.
Osmond, Barry
Raines, Christine A.
von Caemmerer, Susanne
author_facet Heyno, Eiri
Ermakova, Maria
Lopez‐Calcagno, Patricia E.
Woodford, Russell
Brown, Kenny L.
Matthews, Jack S. A.
Osmond, Barry
Raines, Christine A.
von Caemmerer, Susanne
author_sort Heyno, Eiri
collection PubMed
description Photosynthesis is fundamental for plant growth and yield. The cytochrome b (6) f complex catalyses a rate‐limiting step in thylakoid electron transport and therefore represents an important point of regulation of photosynthesis. Here we show that overexpression of a single core subunit of cytochrome b (6) f, the Rieske FeS protein, led to up to a 40% increase in the abundance of the complex in Nicotiana tabacum (tobacco) and was accompanied by an enhanced in vitro cytochrome f activity, indicating a full functionality of the complex. Analysis of transgenic plants overexpressing Rieske FeS by the light‐induced fluorescence transients technique revealed a more oxidised primary quinone acceptor of photosystem II (Q ( A )) and plastoquinone pool and faster electron transport from the plastoquinone pool to photosystem I upon changes in irradiance, compared to control plants. A faster establishment of q ( E ), the energy‐dependent component of nonphotochemical quenching, in transgenic plants suggests a more rapid buildup of the transmembrane proton gradient, also supporting the increased in vivo cytochrome b (6) f activity. However, there was no consistent increase in steady‐state rates of electron transport or CO(2) assimilation in plants overexpressing Rieske FeS grown in either laboratory conditions or field trials, suggesting that the in vivo activity of the complex was only transiently increased upon changes in irradiance. Our results show that overexpression of Rieske FeS in tobacco enhances the abundance of functional cytochrome b (6) f and may have the potential to increase plant productivity if combined with other traits.
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spelling pubmed-98286492023-01-10 Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b (6) f Heyno, Eiri Ermakova, Maria Lopez‐Calcagno, Patricia E. Woodford, Russell Brown, Kenny L. Matthews, Jack S. A. Osmond, Barry Raines, Christine A. von Caemmerer, Susanne Physiol Plant Original Research Photosynthesis is fundamental for plant growth and yield. The cytochrome b (6) f complex catalyses a rate‐limiting step in thylakoid electron transport and therefore represents an important point of regulation of photosynthesis. Here we show that overexpression of a single core subunit of cytochrome b (6) f, the Rieske FeS protein, led to up to a 40% increase in the abundance of the complex in Nicotiana tabacum (tobacco) and was accompanied by an enhanced in vitro cytochrome f activity, indicating a full functionality of the complex. Analysis of transgenic plants overexpressing Rieske FeS by the light‐induced fluorescence transients technique revealed a more oxidised primary quinone acceptor of photosystem II (Q ( A )) and plastoquinone pool and faster electron transport from the plastoquinone pool to photosystem I upon changes in irradiance, compared to control plants. A faster establishment of q ( E ), the energy‐dependent component of nonphotochemical quenching, in transgenic plants suggests a more rapid buildup of the transmembrane proton gradient, also supporting the increased in vivo cytochrome b (6) f activity. However, there was no consistent increase in steady‐state rates of electron transport or CO(2) assimilation in plants overexpressing Rieske FeS grown in either laboratory conditions or field trials, suggesting that the in vivo activity of the complex was only transiently increased upon changes in irradiance. Our results show that overexpression of Rieske FeS in tobacco enhances the abundance of functional cytochrome b (6) f and may have the potential to increase plant productivity if combined with other traits. Blackwell Publishing Ltd 2022-11-04 2022 /pmc/articles/PMC9828649/ /pubmed/36259085 http://dx.doi.org/10.1111/ppl.13803 Text en © 2022 The Authors. Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Heyno, Eiri
Ermakova, Maria
Lopez‐Calcagno, Patricia E.
Woodford, Russell
Brown, Kenny L.
Matthews, Jack S. A.
Osmond, Barry
Raines, Christine A.
von Caemmerer, Susanne
Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b (6) f
title Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b (6) f
title_full Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b (6) f
title_fullStr Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b (6) f
title_full_unstemmed Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b (6) f
title_short Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b (6) f
title_sort rieske fes overexpression in tobacco provides increased abundance and activity of cytochrome b (6) f
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828649/
https://www.ncbi.nlm.nih.gov/pubmed/36259085
http://dx.doi.org/10.1111/ppl.13803
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