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AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus

AdipoRon is an oral active synthetic small molecule with biological functions similar to adiponectin (APN). It is an APN receptor agonist that can improve insulin resistance and glucose intolerance. However, the role of AdipoRon in bone metabolism and related molecular mechanisms remains to be inves...

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Autores principales: Huang, BoRui, Bi, Wei, Sun, Yang, Li, Ruixue, Wu, Xingwen, Yu, Youcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829077/
https://www.ncbi.nlm.nih.gov/pubmed/36632609
http://dx.doi.org/10.3389/fphys.2021.697738
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author Huang, BoRui
Bi, Wei
Sun, Yang
Li, Ruixue
Wu, Xingwen
Yu, Youcheng
author_facet Huang, BoRui
Bi, Wei
Sun, Yang
Li, Ruixue
Wu, Xingwen
Yu, Youcheng
author_sort Huang, BoRui
collection PubMed
description AdipoRon is an oral active synthetic small molecule with biological functions similar to adiponectin (APN). It is an APN receptor agonist that can improve insulin resistance and glucose intolerance. However, the role of AdipoRon in bone metabolism and related molecular mechanisms remains to be investigated. To explore the effect of AdipoRon on bone absorption and bone integration of type 2 diabetes mellitus (T2DM) mice with implants, we established surgery-induced model of osseointegration of dental implantation in T2DM mice of C57BL/6 db/db and normal mice homologous to diabetic mice. Micro-CT was used to analyze the femurs with the implant in the mice to detect the bone mass, H&E, and tartrate-resistant acid phosphatase (TRAP), and Safranin O-fast green staining was performed to analyze the bone formation and bone resorption. Bone integration-related markers as Rankl, bone morphogenetic protein 2 (BMP2), osteoprotegerin (OPG), osteopontin (OPN), and runt-related transcription factor 2 (Runx2) were also measured using immunohistochemistry. Our results indicated that diabetic mice showed a lower bone mass and decreased the osteoblast differentiation. AdipoRon attenuated diabetes-impaired bone volume (BV)/total volume (TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp), and bone integration-related markers variation and promoted bone hyperplasia as well as repressed the osteoclast formation, especially in diabetic mice. AdipoRon may improve the osseointegration of dental implants in mice with T2DM by promoting osteogenesis and inhibiting bone resorption, and AdipoRon may serve as a promising oral strategy to improve the osseointegration ability of patients with diabetes.
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spelling pubmed-98290772023-01-10 AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus Huang, BoRui Bi, Wei Sun, Yang Li, Ruixue Wu, Xingwen Yu, Youcheng Front Physiol Physiology AdipoRon is an oral active synthetic small molecule with biological functions similar to adiponectin (APN). It is an APN receptor agonist that can improve insulin resistance and glucose intolerance. However, the role of AdipoRon in bone metabolism and related molecular mechanisms remains to be investigated. To explore the effect of AdipoRon on bone absorption and bone integration of type 2 diabetes mellitus (T2DM) mice with implants, we established surgery-induced model of osseointegration of dental implantation in T2DM mice of C57BL/6 db/db and normal mice homologous to diabetic mice. Micro-CT was used to analyze the femurs with the implant in the mice to detect the bone mass, H&E, and tartrate-resistant acid phosphatase (TRAP), and Safranin O-fast green staining was performed to analyze the bone formation and bone resorption. Bone integration-related markers as Rankl, bone morphogenetic protein 2 (BMP2), osteoprotegerin (OPG), osteopontin (OPN), and runt-related transcription factor 2 (Runx2) were also measured using immunohistochemistry. Our results indicated that diabetic mice showed a lower bone mass and decreased the osteoblast differentiation. AdipoRon attenuated diabetes-impaired bone volume (BV)/total volume (TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp), and bone integration-related markers variation and promoted bone hyperplasia as well as repressed the osteoclast formation, especially in diabetic mice. AdipoRon may improve the osseointegration of dental implants in mice with T2DM by promoting osteogenesis and inhibiting bone resorption, and AdipoRon may serve as a promising oral strategy to improve the osseointegration ability of patients with diabetes. Frontiers Media S.A. 2021-09-09 /pmc/articles/PMC9829077/ /pubmed/36632609 http://dx.doi.org/10.3389/fphys.2021.697738 Text en Copyright © 2021 Huang, Bi, Sun, Li, Wu and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Huang, BoRui
Bi, Wei
Sun, Yang
Li, Ruixue
Wu, Xingwen
Yu, Youcheng
AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus
title AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus
title_full AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus
title_fullStr AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus
title_full_unstemmed AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus
title_short AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus
title_sort adiporon promotes the osseointegration of dental implants in mice with type 2 diabetes mellitus
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829077/
https://www.ncbi.nlm.nih.gov/pubmed/36632609
http://dx.doi.org/10.3389/fphys.2021.697738
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