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Validation of the efficacy of air purifiers using molecular techniques
The importance of air purifiers has increased in recent years, especially with the “coronavirus disease 2019” pandemic. The efficacy of air purifiers is usually determined under laboratory conditions before widespread application. The standard procedure for testing depends on virus cultivation and t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829175/ https://www.ncbi.nlm.nih.gov/pubmed/36622844 http://dx.doi.org/10.1371/journal.pone.0280243 |
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author | Rausch, Finja Tanneberger, Franziska Abd El Wahed, Ahmed Truyen, Uwe |
author_facet | Rausch, Finja Tanneberger, Franziska Abd El Wahed, Ahmed Truyen, Uwe |
author_sort | Rausch, Finja |
collection | PubMed |
description | The importance of air purifiers has increased in recent years, especially with the “coronavirus disease 2019” pandemic. The efficacy of air purifiers is usually determined under laboratory conditions before widespread application. The standard procedure for testing depends on virus cultivation and titration on cell culture. This, however, requires several days to deliver results. The aim of this study was to establish a rapid molecular assay which can differentiate between intact infectious and distorted non-infectious virus particles. Feline Coronavirus was selected as model for screening. First the samples were pretreated with enzymes (universal nuclease and RNase cocktail enzyme mixture) or viability dye (propidium monoazide) to eliminate any free nucleic acids. The ribonucleic acid (RNA) from intact virus was released via magnetic beads-based extraction, then the amount of the RNA was determined using real-time reverse transcription polymerase chain reaction (RT-PCR) or reverse transcription recombinase-aided amplification (RT-RAA). All results were compared to the infectivity assay based on the calculation of the 50% tissue culture infectious dose (TCID(50)). The nuclease has eliminated 100% of the free Feline Coronavirus RNA, while propidium monoazide underperformed (2.3-fold decrease in free RNA). Both RT-RAA and real-time RT-PCR produced similar results to the infectivity assay on cell culture with limit of detection of 10(2) TCID(50)/mL. Two UV-C air purifiers with prosperities of 100% inactivation of the viruses were used to validate the established procedure. Both real-time RT-PCR and RT-RAA were able to differentiate between intact virus particles and free RNA. To conclude, this study revealed a promising rapid method to validate the efficacy of air purifiers by combining enzymatic pretreatment and molecular assays. |
format | Online Article Text |
id | pubmed-9829175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-98291752023-01-10 Validation of the efficacy of air purifiers using molecular techniques Rausch, Finja Tanneberger, Franziska Abd El Wahed, Ahmed Truyen, Uwe PLoS One Research Article The importance of air purifiers has increased in recent years, especially with the “coronavirus disease 2019” pandemic. The efficacy of air purifiers is usually determined under laboratory conditions before widespread application. The standard procedure for testing depends on virus cultivation and titration on cell culture. This, however, requires several days to deliver results. The aim of this study was to establish a rapid molecular assay which can differentiate between intact infectious and distorted non-infectious virus particles. Feline Coronavirus was selected as model for screening. First the samples were pretreated with enzymes (universal nuclease and RNase cocktail enzyme mixture) or viability dye (propidium monoazide) to eliminate any free nucleic acids. The ribonucleic acid (RNA) from intact virus was released via magnetic beads-based extraction, then the amount of the RNA was determined using real-time reverse transcription polymerase chain reaction (RT-PCR) or reverse transcription recombinase-aided amplification (RT-RAA). All results were compared to the infectivity assay based on the calculation of the 50% tissue culture infectious dose (TCID(50)). The nuclease has eliminated 100% of the free Feline Coronavirus RNA, while propidium monoazide underperformed (2.3-fold decrease in free RNA). Both RT-RAA and real-time RT-PCR produced similar results to the infectivity assay on cell culture with limit of detection of 10(2) TCID(50)/mL. Two UV-C air purifiers with prosperities of 100% inactivation of the viruses were used to validate the established procedure. Both real-time RT-PCR and RT-RAA were able to differentiate between intact virus particles and free RNA. To conclude, this study revealed a promising rapid method to validate the efficacy of air purifiers by combining enzymatic pretreatment and molecular assays. Public Library of Science 2023-01-09 /pmc/articles/PMC9829175/ /pubmed/36622844 http://dx.doi.org/10.1371/journal.pone.0280243 Text en © 2023 Rausch et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rausch, Finja Tanneberger, Franziska Abd El Wahed, Ahmed Truyen, Uwe Validation of the efficacy of air purifiers using molecular techniques |
title | Validation of the efficacy of air purifiers using molecular techniques |
title_full | Validation of the efficacy of air purifiers using molecular techniques |
title_fullStr | Validation of the efficacy of air purifiers using molecular techniques |
title_full_unstemmed | Validation of the efficacy of air purifiers using molecular techniques |
title_short | Validation of the efficacy of air purifiers using molecular techniques |
title_sort | validation of the efficacy of air purifiers using molecular techniques |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829175/ https://www.ncbi.nlm.nih.gov/pubmed/36622844 http://dx.doi.org/10.1371/journal.pone.0280243 |
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