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Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats

PURPOSE: To investigate the role of renal denervation (RDN) on endogenous ouabain (EO) secretion in spontaneously hypertensive rats (SHR). METHODS: Sixteen 12-week-old male SHR were randomly separated into the renal denervation group (RDNX group) and sham operation group (sham group), and eight age-...

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Autores principales: Lai, Xiaomei, Wen, Hong, Yang, Tingting, Qin, Fei, Zhong, Xiaoge, Pan, Yajin, Yu, Jie, Huang, Jing, Li, Jianling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829196/
https://www.ncbi.nlm.nih.gov/pubmed/36629529
http://dx.doi.org/10.1590/acb371102
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author Lai, Xiaomei
Wen, Hong
Yang, Tingting
Qin, Fei
Zhong, Xiaoge
Pan, Yajin
Yu, Jie
Huang, Jing
Li, Jianling
author_facet Lai, Xiaomei
Wen, Hong
Yang, Tingting
Qin, Fei
Zhong, Xiaoge
Pan, Yajin
Yu, Jie
Huang, Jing
Li, Jianling
author_sort Lai, Xiaomei
collection PubMed
description PURPOSE: To investigate the role of renal denervation (RDN) on endogenous ouabain (EO) secretion in spontaneously hypertensive rats (SHR). METHODS: Sixteen 12-week-old male SHR were randomly separated into the renal denervation group (RDNX group) and sham operation group (sham group), and eight age-matched Wistar Kyoto rats (WKY) were served as control group. EO concentrations, the Na(+)- K(+)-ATPaseactivity, and the expression of Na(+)-K(+)-ATPase were assessed. RESULTS: EO levels in serum, kidneys and hypothalamus of sham group were higher than in RDNX group (p < 0.05). Renal Na(+)-K(+)-ATPase activity subjected to denervation surgery showed significantly reduction when compared with the sham groups (p < 0.05). A positive correlation existed between norepinephrine (NE) content and Na(+)-K(+)-ATPase activity in the kidney (r(2) = 0.579). Renal Na(+)-K(+)-ATPase α1 subunit mRNA expression was down-regulated in the RDNX group compared with the sham group (P < 0.05), while renal Na(+)-K(+)-ATPase α1 subunit mRNA expression was no statistical significance between the groups (P = 0.63). Immunohistochemical analysis showed that there were significant differences in the renal expression of Na(+)-K(+)-ATPasebetween the three groups (P < 0.05). CONCLUSIONS: These experiments demonstrate that RDN exerted an anti-hypertensive effect with reduction of EO levels and Na(+)-K(+)-ATPase activity and Na(+)-K(+)-ATPase α1 subunit expression of kidney in SHR.
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spelling pubmed-98291962023-01-10 Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats Lai, Xiaomei Wen, Hong Yang, Tingting Qin, Fei Zhong, Xiaoge Pan, Yajin Yu, Jie Huang, Jing Li, Jianling Acta Cir Bras Original Article PURPOSE: To investigate the role of renal denervation (RDN) on endogenous ouabain (EO) secretion in spontaneously hypertensive rats (SHR). METHODS: Sixteen 12-week-old male SHR were randomly separated into the renal denervation group (RDNX group) and sham operation group (sham group), and eight age-matched Wistar Kyoto rats (WKY) were served as control group. EO concentrations, the Na(+)- K(+)-ATPaseactivity, and the expression of Na(+)-K(+)-ATPase were assessed. RESULTS: EO levels in serum, kidneys and hypothalamus of sham group were higher than in RDNX group (p < 0.05). Renal Na(+)-K(+)-ATPase activity subjected to denervation surgery showed significantly reduction when compared with the sham groups (p < 0.05). A positive correlation existed between norepinephrine (NE) content and Na(+)-K(+)-ATPase activity in the kidney (r(2) = 0.579). Renal Na(+)-K(+)-ATPase α1 subunit mRNA expression was down-regulated in the RDNX group compared with the sham group (P < 0.05), while renal Na(+)-K(+)-ATPase α1 subunit mRNA expression was no statistical significance between the groups (P = 0.63). Immunohistochemical analysis showed that there were significant differences in the renal expression of Na(+)-K(+)-ATPasebetween the three groups (P < 0.05). CONCLUSIONS: These experiments demonstrate that RDN exerted an anti-hypertensive effect with reduction of EO levels and Na(+)-K(+)-ATPase activity and Na(+)-K(+)-ATPase α1 subunit expression of kidney in SHR. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2023-01-06 /pmc/articles/PMC9829196/ /pubmed/36629529 http://dx.doi.org/10.1590/acb371102 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lai, Xiaomei
Wen, Hong
Yang, Tingting
Qin, Fei
Zhong, Xiaoge
Pan, Yajin
Yu, Jie
Huang, Jing
Li, Jianling
Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats
title Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats
title_full Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats
title_fullStr Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats
title_full_unstemmed Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats
title_short Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats
title_sort effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829196/
https://www.ncbi.nlm.nih.gov/pubmed/36629529
http://dx.doi.org/10.1590/acb371102
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