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SPINDLY interacts with EIN2 to facilitate ethylene signalling‐mediated fruit ripening in tomato

The post‐translational modification of proteins enables cells to respond promptly to dynamic stimuli by controlling protein functions. In higher plants, SPINDLY (SPY) and SECRET AGENT (SEC) are two prominent O‐glycosylation enzymes that have both unique and overlapping roles; however, the effects of...

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Autores principales: Xu, Jin, Liu, Sidi, Cai, Licong, Wang, Lingyu, Dong, Yufei, Qi, Zhenyu, Yu, Jingquan, Zhou, Yanhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829397/
https://www.ncbi.nlm.nih.gov/pubmed/36204970
http://dx.doi.org/10.1111/pbi.13939
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author Xu, Jin
Liu, Sidi
Cai, Licong
Wang, Lingyu
Dong, Yufei
Qi, Zhenyu
Yu, Jingquan
Zhou, Yanhong
author_facet Xu, Jin
Liu, Sidi
Cai, Licong
Wang, Lingyu
Dong, Yufei
Qi, Zhenyu
Yu, Jingquan
Zhou, Yanhong
author_sort Xu, Jin
collection PubMed
description The post‐translational modification of proteins enables cells to respond promptly to dynamic stimuli by controlling protein functions. In higher plants, SPINDLY (SPY) and SECRET AGENT (SEC) are two prominent O‐glycosylation enzymes that have both unique and overlapping roles; however, the effects of their O‐glycosylation on fruit ripening and the underlying mechanisms remain largely unknown. Here we report that SlSPY affects tomato fruit ripening. Using slspy mutants and two SlSPY‐OE lines, we provide biological evidence for the positive role of SlSPY in fruit ripening. We demonstrate that SlSPY regulates fruit ripening by changing the ethylene response in tomato. To further investigate the underlying mechanism, we identify a central regulator of ethylene signalling ETHYLENE INSENSITIVE 2 (EIN2) as a SlSPY interacting protein. SlSPY promotes the stability and nuclear accumulation of SlEIN2. Mass spectrometry analysis further identified that SlEIN2 has two potential sites Ser771 and Thr821 of O‐glycans modifications. Further study shows that SlEIN2 is essential for SlSPY in regulating fruit ripening in tomatoes. Collectively, our findings reveal a novel regulatory function of SlSPY in fruit and provide novel insights into the role of the SlSPY‐SlEIN2 module in tomato fruit ripening.
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spelling pubmed-98293972023-01-10 SPINDLY interacts with EIN2 to facilitate ethylene signalling‐mediated fruit ripening in tomato Xu, Jin Liu, Sidi Cai, Licong Wang, Lingyu Dong, Yufei Qi, Zhenyu Yu, Jingquan Zhou, Yanhong Plant Biotechnol J Research Articles The post‐translational modification of proteins enables cells to respond promptly to dynamic stimuli by controlling protein functions. In higher plants, SPINDLY (SPY) and SECRET AGENT (SEC) are two prominent O‐glycosylation enzymes that have both unique and overlapping roles; however, the effects of their O‐glycosylation on fruit ripening and the underlying mechanisms remain largely unknown. Here we report that SlSPY affects tomato fruit ripening. Using slspy mutants and two SlSPY‐OE lines, we provide biological evidence for the positive role of SlSPY in fruit ripening. We demonstrate that SlSPY regulates fruit ripening by changing the ethylene response in tomato. To further investigate the underlying mechanism, we identify a central regulator of ethylene signalling ETHYLENE INSENSITIVE 2 (EIN2) as a SlSPY interacting protein. SlSPY promotes the stability and nuclear accumulation of SlEIN2. Mass spectrometry analysis further identified that SlEIN2 has two potential sites Ser771 and Thr821 of O‐glycans modifications. Further study shows that SlEIN2 is essential for SlSPY in regulating fruit ripening in tomatoes. Collectively, our findings reveal a novel regulatory function of SlSPY in fruit and provide novel insights into the role of the SlSPY‐SlEIN2 module in tomato fruit ripening. John Wiley and Sons Inc. 2022-11-02 2023-01 /pmc/articles/PMC9829397/ /pubmed/36204970 http://dx.doi.org/10.1111/pbi.13939 Text en © 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Xu, Jin
Liu, Sidi
Cai, Licong
Wang, Lingyu
Dong, Yufei
Qi, Zhenyu
Yu, Jingquan
Zhou, Yanhong
SPINDLY interacts with EIN2 to facilitate ethylene signalling‐mediated fruit ripening in tomato
title SPINDLY interacts with EIN2 to facilitate ethylene signalling‐mediated fruit ripening in tomato
title_full SPINDLY interacts with EIN2 to facilitate ethylene signalling‐mediated fruit ripening in tomato
title_fullStr SPINDLY interacts with EIN2 to facilitate ethylene signalling‐mediated fruit ripening in tomato
title_full_unstemmed SPINDLY interacts with EIN2 to facilitate ethylene signalling‐mediated fruit ripening in tomato
title_short SPINDLY interacts with EIN2 to facilitate ethylene signalling‐mediated fruit ripening in tomato
title_sort spindly interacts with ein2 to facilitate ethylene signalling‐mediated fruit ripening in tomato
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829397/
https://www.ncbi.nlm.nih.gov/pubmed/36204970
http://dx.doi.org/10.1111/pbi.13939
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