Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biology, genetics, and biotechnology, but wider deploy...

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Detalles Bibliográficos
Autores principales: Replogle, Joseph M, Bonnar, Jessica L, Pogson, Angela N, Liem, Christina R, Maier, Nolan K, Ding, Yufang, Russell, Baylee J, Wang, Xingren, Leng, Kun, Guna, Alina, Norman, Thomas M, Pak, Ryan A, Ramos, Daniel M, Ward, Michael E, Gilbert, Luke A, Kampmann, Martin, Weissman, Jonathan S, Jost, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Genetics and Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829409/
https://www.ncbi.nlm.nih.gov/pubmed/36576240
http://dx.doi.org/10.7554/eLife.81856

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