Attenuation of hexaconazole induced oxidative stress by folic acid, malic acid and ferrocenecarboxaldehyde in an invertebrate model Bombyxmori

Fungicides are a class of pesticides used to ward off fungal diseases from agricultural crops to achieve maximum productivity. These chemicals are quite efficient in controlling diseases; however, the excessive use of these affects non-target organisms as well. In this study, Bombyx mori was utilized to investigate the effect of the pesticide hexaconazole (HEX) on the antioxidant system of this organism and also to find ways to mitigate it. On oral exposure to this chemical, a significant reduction in antioxidants, CAT, GPX, GSH, and SOD in the gut, fat body, and silk gland was observed. The HEX treatment also resulted in lipid peroxidation (LPO) in all the three tissues. To mitigate this toxicity and protect the silkworm from oxidative stress, we tested three compounds, namely folic acid, ferrocenecarboxaldehyde, and malic acid having known antioxidant potential. Folic acid provided significant protection against HEX-induced toxicity. Ferrocenecarboxaldehyde and malic acid proved to be ill-efficient in controlling oxidative stress, with ferrocenecarboxaldehyde being the least effective of the three. Folic acid was also efficient in controlling LPO up to a considerable level. Ferrocenecarboxaldehyde and malic acid also prevented LPO less efficiently than folic acid. Overall folic acid was the only compound that mitigated HEX-induced oxidative stress in silkworm with statistical significance in all the tissues viz. gut, fat body, and silk gland.