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Age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs
BACKGROUND: The pig gastrointestinal tract hosts a diverse microbiome, which can serve to select and maintain a reservoir of antimicrobial resistance genes (ARG). Studies suggest that the types and quantities of antimicrobial resistance (AMR) in fecal bacteria change as the animal host ages, yet the...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9830919/ https://www.ncbi.nlm.nih.gov/pubmed/36624546 http://dx.doi.org/10.1186/s42523-022-00222-8 |
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author | Gaire, Tara N. Scott, H. Morgan Noyes, Noelle R. Ericsson, Aaron C. Tokach, Michael D. Menegat, Mariana B. Vinasco, Javier Roenne, Boyd Ray, Tui Nagaraja, T. G. Volkova, Victoriya V. |
author_facet | Gaire, Tara N. Scott, H. Morgan Noyes, Noelle R. Ericsson, Aaron C. Tokach, Michael D. Menegat, Mariana B. Vinasco, Javier Roenne, Boyd Ray, Tui Nagaraja, T. G. Volkova, Victoriya V. |
author_sort | Gaire, Tara N. |
collection | PubMed |
description | BACKGROUND: The pig gastrointestinal tract hosts a diverse microbiome, which can serve to select and maintain a reservoir of antimicrobial resistance genes (ARG). Studies suggest that the types and quantities of antimicrobial resistance (AMR) in fecal bacteria change as the animal host ages, yet the temporal dynamics of AMR within communities of bacteria in pigs during a full production cycle remains largely unstudied. RESULTS: A longitudinal study was performed to evaluate the dynamics of fecal microbiome and AMR in a cohort of pigs during a production cycle; from birth to market age. Our data showed that piglet fecal microbial communities assemble rapidly after birth and become more diverse with age. Individual piglet fecal microbiomes progressed along similar trajectories with age-specific community types/enterotypes and showed a clear shift from E. coli/Shigella-, Fusobacteria-, Bacteroides-dominant enterotypes to Prevotella-, Megaspheara-, and Lactobacillus-dominated enterotypes with aging. Even when the fecal microbiome was the least diverse, the richness of ARGs, quantities of AMR gene copies, and counts of AMR fecal bacteria were highest in piglets at 2 days of age; subsequently, these declined over time, likely due to age-related competitive changes in the underlying microbiome. ARGs conferring resistance to metals and multi-compound/biocides were detected predominately at the earliest sampled ages. CONCLUSIONS: The fecal microbiome and resistome—along with evaluated descriptors of phenotypic antimicrobial susceptibility of fecal bacteria—among a cohort of pigs, demonstrated opposing trajectories in diversity primarily driven by the aging of pigs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-022-00222-8. |
format | Online Article Text |
id | pubmed-9830919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-98309192023-01-11 Age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs Gaire, Tara N. Scott, H. Morgan Noyes, Noelle R. Ericsson, Aaron C. Tokach, Michael D. Menegat, Mariana B. Vinasco, Javier Roenne, Boyd Ray, Tui Nagaraja, T. G. Volkova, Victoriya V. Anim Microbiome Research BACKGROUND: The pig gastrointestinal tract hosts a diverse microbiome, which can serve to select and maintain a reservoir of antimicrobial resistance genes (ARG). Studies suggest that the types and quantities of antimicrobial resistance (AMR) in fecal bacteria change as the animal host ages, yet the temporal dynamics of AMR within communities of bacteria in pigs during a full production cycle remains largely unstudied. RESULTS: A longitudinal study was performed to evaluate the dynamics of fecal microbiome and AMR in a cohort of pigs during a production cycle; from birth to market age. Our data showed that piglet fecal microbial communities assemble rapidly after birth and become more diverse with age. Individual piglet fecal microbiomes progressed along similar trajectories with age-specific community types/enterotypes and showed a clear shift from E. coli/Shigella-, Fusobacteria-, Bacteroides-dominant enterotypes to Prevotella-, Megaspheara-, and Lactobacillus-dominated enterotypes with aging. Even when the fecal microbiome was the least diverse, the richness of ARGs, quantities of AMR gene copies, and counts of AMR fecal bacteria were highest in piglets at 2 days of age; subsequently, these declined over time, likely due to age-related competitive changes in the underlying microbiome. ARGs conferring resistance to metals and multi-compound/biocides were detected predominately at the earliest sampled ages. CONCLUSIONS: The fecal microbiome and resistome—along with evaluated descriptors of phenotypic antimicrobial susceptibility of fecal bacteria—among a cohort of pigs, demonstrated opposing trajectories in diversity primarily driven by the aging of pigs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-022-00222-8. BioMed Central 2023-01-10 /pmc/articles/PMC9830919/ /pubmed/36624546 http://dx.doi.org/10.1186/s42523-022-00222-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Gaire, Tara N. Scott, H. Morgan Noyes, Noelle R. Ericsson, Aaron C. Tokach, Michael D. Menegat, Mariana B. Vinasco, Javier Roenne, Boyd Ray, Tui Nagaraja, T. G. Volkova, Victoriya V. Age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs |
title | Age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs |
title_full | Age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs |
title_fullStr | Age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs |
title_full_unstemmed | Age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs |
title_short | Age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs |
title_sort | age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9830919/ https://www.ncbi.nlm.nih.gov/pubmed/36624546 http://dx.doi.org/10.1186/s42523-022-00222-8 |
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