Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)

Epstein-Barr virus (EBV) immortalized lymphoblastoid cell lines (LCLs) are widely used for banking. This bioresource could be leveraged for creating human iPSC lines to model diseases including CHD. We generated an LCL-derived iPSC line (NCHi001-A) from a patient with congenital aortic valve stenosi...

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Autores principales: Lin, Hui, Ye, Shi-Qiao, Xu, Zhao-Hui, Penaloza, Jacqueline S., Aljuhani, Mona, Vetter, Tatyana, Zhao, Ming-Tao, McBride, Kim L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831069/
https://www.ncbi.nlm.nih.gov/pubmed/36343514
http://dx.doi.org/10.1016/j.scr.2022.102958
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author Lin, Hui
Ye, Shi-Qiao
Xu, Zhao-Hui
Penaloza, Jacqueline S.
Aljuhani, Mona
Vetter, Tatyana
Zhao, Ming-Tao
McBride, Kim L.
author_facet Lin, Hui
Ye, Shi-Qiao
Xu, Zhao-Hui
Penaloza, Jacqueline S.
Aljuhani, Mona
Vetter, Tatyana
Zhao, Ming-Tao
McBride, Kim L.
author_sort Lin, Hui
collection PubMed
description Epstein-Barr virus (EBV) immortalized lymphoblastoid cell lines (LCLs) are widely used for banking. This bioresource could be leveraged for creating human iPSC lines to model diseases including CHD. We generated an LCL-derived iPSC line (NCHi001-A) from a patient with congenital aortic valve stenosis. NCHi001-A was EBV and transgenes free, exhibited stem cell-like morphology, expressed pluripotency markers, has a normal karyotype, and could be differentiated into cells of three germ layers in vitro. Relationship inference via a microarray-based analysis showed NCHi001-A is identical to the parental cell line. NCHi001-A can be used for disease modeling, drug discovery, and cell therapy development.
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spelling pubmed-98310692023-01-10 Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD) Lin, Hui Ye, Shi-Qiao Xu, Zhao-Hui Penaloza, Jacqueline S. Aljuhani, Mona Vetter, Tatyana Zhao, Ming-Tao McBride, Kim L. Stem Cell Res Article Epstein-Barr virus (EBV) immortalized lymphoblastoid cell lines (LCLs) are widely used for banking. This bioresource could be leveraged for creating human iPSC lines to model diseases including CHD. We generated an LCL-derived iPSC line (NCHi001-A) from a patient with congenital aortic valve stenosis. NCHi001-A was EBV and transgenes free, exhibited stem cell-like morphology, expressed pluripotency markers, has a normal karyotype, and could be differentiated into cells of three germ layers in vitro. Relationship inference via a microarray-based analysis showed NCHi001-A is identical to the parental cell line. NCHi001-A can be used for disease modeling, drug discovery, and cell therapy development. 2022-12 2022-10-31 /pmc/articles/PMC9831069/ /pubmed/36343514 http://dx.doi.org/10.1016/j.scr.2022.102958 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Lin, Hui
Ye, Shi-Qiao
Xu, Zhao-Hui
Penaloza, Jacqueline S.
Aljuhani, Mona
Vetter, Tatyana
Zhao, Ming-Tao
McBride, Kim L.
Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
title Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
title_full Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
title_fullStr Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
title_full_unstemmed Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
title_short Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
title_sort generation and characterization of a human induced pluripotent stem cell (ipsc) line from a patient with congenital heart disease (chd)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831069/
https://www.ncbi.nlm.nih.gov/pubmed/36343514
http://dx.doi.org/10.1016/j.scr.2022.102958
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