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High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle

Aiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows’ reproductive efficiency, cows were fed with different nutritional plans to obtain animals with high BER (HBER; Ad libitum diet) and moderate BER (MBER: cows fed 70% of HBER group ingestion). To evaluate the BER, c...

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Autores principales: Bastos, Natália Marins, Goulart, Rodrigo Silva, Bambil, Danilo Brito, Bridi, Alessandra, Mazzarella, Rosane, Alves, Luana, da Silva Rosa, Paola Maria, Neto, Adomar Laurindo, Silva, Saulo Luz, de Almeida Santana, Miguel Henrique, Negrão, João Alberto, Pugliesi, Guilherme, Meirelles, Flávio Vieira, Perecin, Felipe, da Silveira, Juliano Coelho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831338/
https://www.ncbi.nlm.nih.gov/pubmed/36626404
http://dx.doi.org/10.1371/journal.pone.0280195
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author Bastos, Natália Marins
Goulart, Rodrigo Silva
Bambil, Danilo Brito
Bridi, Alessandra
Mazzarella, Rosane
Alves, Luana
da Silva Rosa, Paola Maria
Neto, Adomar Laurindo
Silva, Saulo Luz
de Almeida Santana, Miguel Henrique
Negrão, João Alberto
Pugliesi, Guilherme
Meirelles, Flávio Vieira
Perecin, Felipe
da Silveira, Juliano Coelho
author_facet Bastos, Natália Marins
Goulart, Rodrigo Silva
Bambil, Danilo Brito
Bridi, Alessandra
Mazzarella, Rosane
Alves, Luana
da Silva Rosa, Paola Maria
Neto, Adomar Laurindo
Silva, Saulo Luz
de Almeida Santana, Miguel Henrique
Negrão, João Alberto
Pugliesi, Guilherme
Meirelles, Flávio Vieira
Perecin, Felipe
da Silveira, Juliano Coelho
author_sort Bastos, Natália Marins
collection PubMed
description Aiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows’ reproductive efficiency, cows were fed with different nutritional plans to obtain animals with high BER (HBER; Ad libitum diet) and moderate BER (MBER: cows fed 70% of HBER group ingestion). To evaluate the BER, cows were weekly weighted and evaluated for subcutaneous fat thickness and insulin serum concentration along the experimental period. At the end of the experimental period, animals were submitted to estrous synchronization and artificial insemination. Animals were slaughtered approximately 120 h after ovulation induction and the reproductive tracts were collected for embryo recovery and samples collection. Cumulus-oocyte-complexes (COC) and follicular fluid were collected from 3–6 mm in diameter ovarian follicles to perform miRNA analysis of cumulus cells (CC) and extracellular vesicles from follicular fluid (EV FF). As expected, differences were observed among MBER and HBER groups for body weight, fat thickness, and insulin serum concentration. HBER animals showed lower ovulation and embryo recovery rates compared to MBER animals. Different miRNAs were found among CC and EV FF within groups, suggesting that the BER may influence follicular communication. This suggests that small follicles (3–6 mm diameter) are already under BER effects, which may be greater on later stages of follicular development.
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spelling pubmed-98313382023-01-11 High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle Bastos, Natália Marins Goulart, Rodrigo Silva Bambil, Danilo Brito Bridi, Alessandra Mazzarella, Rosane Alves, Luana da Silva Rosa, Paola Maria Neto, Adomar Laurindo Silva, Saulo Luz de Almeida Santana, Miguel Henrique Negrão, João Alberto Pugliesi, Guilherme Meirelles, Flávio Vieira Perecin, Felipe da Silveira, Juliano Coelho PLoS One Research Article Aiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows’ reproductive efficiency, cows were fed with different nutritional plans to obtain animals with high BER (HBER; Ad libitum diet) and moderate BER (MBER: cows fed 70% of HBER group ingestion). To evaluate the BER, cows were weekly weighted and evaluated for subcutaneous fat thickness and insulin serum concentration along the experimental period. At the end of the experimental period, animals were submitted to estrous synchronization and artificial insemination. Animals were slaughtered approximately 120 h after ovulation induction and the reproductive tracts were collected for embryo recovery and samples collection. Cumulus-oocyte-complexes (COC) and follicular fluid were collected from 3–6 mm in diameter ovarian follicles to perform miRNA analysis of cumulus cells (CC) and extracellular vesicles from follicular fluid (EV FF). As expected, differences were observed among MBER and HBER groups for body weight, fat thickness, and insulin serum concentration. HBER animals showed lower ovulation and embryo recovery rates compared to MBER animals. Different miRNAs were found among CC and EV FF within groups, suggesting that the BER may influence follicular communication. This suggests that small follicles (3–6 mm diameter) are already under BER effects, which may be greater on later stages of follicular development. Public Library of Science 2023-01-10 /pmc/articles/PMC9831338/ /pubmed/36626404 http://dx.doi.org/10.1371/journal.pone.0280195 Text en © 2023 Bastos et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bastos, Natália Marins
Goulart, Rodrigo Silva
Bambil, Danilo Brito
Bridi, Alessandra
Mazzarella, Rosane
Alves, Luana
da Silva Rosa, Paola Maria
Neto, Adomar Laurindo
Silva, Saulo Luz
de Almeida Santana, Miguel Henrique
Negrão, João Alberto
Pugliesi, Guilherme
Meirelles, Flávio Vieira
Perecin, Felipe
da Silveira, Juliano Coelho
High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle
title High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle
title_full High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle
title_fullStr High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle
title_full_unstemmed High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle
title_short High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle
title_sort high body energy reserve influences extracellular vesicles mirna contents within the ovarian follicle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831338/
https://www.ncbi.nlm.nih.gov/pubmed/36626404
http://dx.doi.org/10.1371/journal.pone.0280195
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