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Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa

The circadian clock governs rhythmic cellular functions by driving the expression of a substantial fraction of the genome and thereby significantly contributes to the adaptation to changing environmental conditions. Using the circadian model organism Neurospora crassa, we show that molecular timekee...

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Autores principales: Szőke, Anita, Sárkány, Orsolya, Schermann, Géza, Kapuy, Orsolya, Diernfellner, Axel CR, Brunner, Michael, Gyöngyösi, Norbert, Káldi, Krisztina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831608/
https://www.ncbi.nlm.nih.gov/pubmed/36625037
http://dx.doi.org/10.7554/eLife.79765
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author Szőke, Anita
Sárkány, Orsolya
Schermann, Géza
Kapuy, Orsolya
Diernfellner, Axel CR
Brunner, Michael
Gyöngyösi, Norbert
Káldi, Krisztina
author_facet Szőke, Anita
Sárkány, Orsolya
Schermann, Géza
Kapuy, Orsolya
Diernfellner, Axel CR
Brunner, Michael
Gyöngyösi, Norbert
Káldi, Krisztina
author_sort Szőke, Anita
collection PubMed
description The circadian clock governs rhythmic cellular functions by driving the expression of a substantial fraction of the genome and thereby significantly contributes to the adaptation to changing environmental conditions. Using the circadian model organism Neurospora crassa, we show that molecular timekeeping is robust even under severe limitation of carbon sources, however, stoichiometry, phosphorylation and subcellular distribution of the key clock components display drastic alterations. Protein kinase A, protein phosphatase 2 A and glycogen synthase kinase are involved in the molecular reorganization of the clock. RNA-seq analysis reveals that the transcriptomic response of metabolism to starvation is highly dependent on the positive clock component WC-1. Moreover, our molecular and phenotypic data indicate that a functional clock facilitates recovery from starvation. We suggest that the molecular clock is a flexible network that allows the organism to maintain rhythmic physiology and preserve fitness even under long-term nutritional stress.
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spelling pubmed-98316082023-01-11 Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa Szőke, Anita Sárkány, Orsolya Schermann, Géza Kapuy, Orsolya Diernfellner, Axel CR Brunner, Michael Gyöngyösi, Norbert Káldi, Krisztina eLife Cell Biology The circadian clock governs rhythmic cellular functions by driving the expression of a substantial fraction of the genome and thereby significantly contributes to the adaptation to changing environmental conditions. Using the circadian model organism Neurospora crassa, we show that molecular timekeeping is robust even under severe limitation of carbon sources, however, stoichiometry, phosphorylation and subcellular distribution of the key clock components display drastic alterations. Protein kinase A, protein phosphatase 2 A and glycogen synthase kinase are involved in the molecular reorganization of the clock. RNA-seq analysis reveals that the transcriptomic response of metabolism to starvation is highly dependent on the positive clock component WC-1. Moreover, our molecular and phenotypic data indicate that a functional clock facilitates recovery from starvation. We suggest that the molecular clock is a flexible network that allows the organism to maintain rhythmic physiology and preserve fitness even under long-term nutritional stress. eLife Sciences Publications, Ltd 2023-01-10 /pmc/articles/PMC9831608/ /pubmed/36625037 http://dx.doi.org/10.7554/eLife.79765 Text en © 2023, Szőke et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Szőke, Anita
Sárkány, Orsolya
Schermann, Géza
Kapuy, Orsolya
Diernfellner, Axel CR
Brunner, Michael
Gyöngyösi, Norbert
Káldi, Krisztina
Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa
title Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa
title_full Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa
title_fullStr Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa
title_full_unstemmed Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa
title_short Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa
title_sort adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in neurospora crassa
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831608/
https://www.ncbi.nlm.nih.gov/pubmed/36625037
http://dx.doi.org/10.7554/eLife.79765
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