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Comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans
BACKGROUND/PURPOSE: Due to their regenerative potential, periodontal ligament (PDL) and umbilical cord (UBC) tissues are an attractive potential mesenchymal stem cells (MSCs) source. This study compared the expression patterns of genes related to stemness between fresh PDL and UBC tissues. MATERIALS...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Association for Dental Sciences of the Republic of China
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831792/ https://www.ncbi.nlm.nih.gov/pubmed/36643271 http://dx.doi.org/10.1016/j.jds.2022.06.005 |
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author | Lee, Hyung-Joo Jeon, Mijeong Kim, Young-Han Kim, Seong-Oh Lee, Ko Eun |
author_facet | Lee, Hyung-Joo Jeon, Mijeong Kim, Young-Han Kim, Seong-Oh Lee, Ko Eun |
author_sort | Lee, Hyung-Joo |
collection | PubMed |
description | BACKGROUND/PURPOSE: Due to their regenerative potential, periodontal ligament (PDL) and umbilical cord (UBC) tissues are an attractive potential mesenchymal stem cells (MSCs) source. This study compared the expression patterns of genes related to stemness between fresh PDL and UBC tissues. MATERIALS AND METHODS: PDL tissues were collected from 38 permanent premolars extracted for orthodontic purposes, and UBC tissues were obtained from three newborns. Each sample was immediately frozen to prevent RNA degradation. cDNA microarray analysis, quantitative real-time polymerase chain reaction (PCR), and immunohistochemical staining were performed. Gene expression patterns associated with dental stemness (DS) and induced pluripotent stemness (iPS) were compared between PDL and UBC tissues. RESULTS: In the cDNA microarray analyses, the expressions of most iPS genes were greater in the PDL than in the UBC. Meanwhile, the expressions of most DS genes were greater in the UBC than in the PDL. Quantitative real-time PCR analyses showed that the expression levels of matrix metallopeptidase 13 (MMP13), ADAM metallopeptidase domain 22 (ADAM22), vascular cell adhesion protein 1 (VCAM1), and kruppel-like factor 4 (KLF4) genes were greater in the PDL than in the UBC, while the expressions of melanoma cell adhesion molecule (MCAM) and activated leukocyte cell adhesion molecule (ALCAM) were greater in the UBC than in the PDL. CONCLUSION: These results suggest that UBC and PDL tissues showed slightly different expression patterns of genes related to stemness, which warrants further investigation to use these tissues for future regeneration and implantation therapies. |
format | Online Article Text |
id | pubmed-9831792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Association for Dental Sciences of the Republic of China |
record_format | MEDLINE/PubMed |
spelling | pubmed-98317922023-01-13 Comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans Lee, Hyung-Joo Jeon, Mijeong Kim, Young-Han Kim, Seong-Oh Lee, Ko Eun J Dent Sci Original Article BACKGROUND/PURPOSE: Due to their regenerative potential, periodontal ligament (PDL) and umbilical cord (UBC) tissues are an attractive potential mesenchymal stem cells (MSCs) source. This study compared the expression patterns of genes related to stemness between fresh PDL and UBC tissues. MATERIALS AND METHODS: PDL tissues were collected from 38 permanent premolars extracted for orthodontic purposes, and UBC tissues were obtained from three newborns. Each sample was immediately frozen to prevent RNA degradation. cDNA microarray analysis, quantitative real-time polymerase chain reaction (PCR), and immunohistochemical staining were performed. Gene expression patterns associated with dental stemness (DS) and induced pluripotent stemness (iPS) were compared between PDL and UBC tissues. RESULTS: In the cDNA microarray analyses, the expressions of most iPS genes were greater in the PDL than in the UBC. Meanwhile, the expressions of most DS genes were greater in the UBC than in the PDL. Quantitative real-time PCR analyses showed that the expression levels of matrix metallopeptidase 13 (MMP13), ADAM metallopeptidase domain 22 (ADAM22), vascular cell adhesion protein 1 (VCAM1), and kruppel-like factor 4 (KLF4) genes were greater in the PDL than in the UBC, while the expressions of melanoma cell adhesion molecule (MCAM) and activated leukocyte cell adhesion molecule (ALCAM) were greater in the UBC than in the PDL. CONCLUSION: These results suggest that UBC and PDL tissues showed slightly different expression patterns of genes related to stemness, which warrants further investigation to use these tissues for future regeneration and implantation therapies. Association for Dental Sciences of the Republic of China 2023-01 2022-06-29 /pmc/articles/PMC9831792/ /pubmed/36643271 http://dx.doi.org/10.1016/j.jds.2022.06.005 Text en © 2022 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Lee, Hyung-Joo Jeon, Mijeong Kim, Young-Han Kim, Seong-Oh Lee, Ko Eun Comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans |
title | Comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans |
title_full | Comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans |
title_fullStr | Comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans |
title_full_unstemmed | Comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans |
title_short | Comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans |
title_sort | comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831792/ https://www.ncbi.nlm.nih.gov/pubmed/36643271 http://dx.doi.org/10.1016/j.jds.2022.06.005 |
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