Changes of lysosome by L-serine in rotenone-treated hippocampal neurons

Oxidative stress destroys cellular organelles and damages DNA, eventually leading to degenerative brain disorders. Persistent mitochondrial damage by oxidative stress eventually causes cells to inhibit the function of lysosomes. Rotenone used in this study inhibits complex 1 of the mitochondrial ele...

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Autores principales: Shin, Sumin, Hwang, Su-Kyeong, Mun, Ji Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832202/
https://www.ncbi.nlm.nih.gov/pubmed/36626017
http://dx.doi.org/10.1186/s42649-022-00084-z
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author Shin, Sumin
Hwang, Su-Kyeong
Mun, Ji Young
author_facet Shin, Sumin
Hwang, Su-Kyeong
Mun, Ji Young
author_sort Shin, Sumin
collection PubMed
description Oxidative stress destroys cellular organelles and damages DNA, eventually leading to degenerative brain disorders. Persistent mitochondrial damage by oxidative stress eventually causes cells to inhibit the function of lysosomes. Rotenone used in this study inhibits complex 1 of the mitochondrial electron transport chain. Due to this inhibition, the production of free radicals is promoted, and oxidative stress can occur. To test as a role of antioxidant, L-serine was treated before treatment of rotenone to HT22 hippocampal cells. Then, changes in the activity and structure of lysosomes were analyzed. As a result, the oxidative stress caused by rotenone in HT22 cells was protected by L-serine. L-serine reduced free radicals in cells, and the damaged lysosomal structure and lysosome activity were also protected.
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spelling pubmed-98322022023-01-12 Changes of lysosome by L-serine in rotenone-treated hippocampal neurons Shin, Sumin Hwang, Su-Kyeong Mun, Ji Young Appl Microsc Research Oxidative stress destroys cellular organelles and damages DNA, eventually leading to degenerative brain disorders. Persistent mitochondrial damage by oxidative stress eventually causes cells to inhibit the function of lysosomes. Rotenone used in this study inhibits complex 1 of the mitochondrial electron transport chain. Due to this inhibition, the production of free radicals is promoted, and oxidative stress can occur. To test as a role of antioxidant, L-serine was treated before treatment of rotenone to HT22 hippocampal cells. Then, changes in the activity and structure of lysosomes were analyzed. As a result, the oxidative stress caused by rotenone in HT22 cells was protected by L-serine. L-serine reduced free radicals in cells, and the damaged lysosomal structure and lysosome activity were also protected. Springer Nature Singapore 2023-01-10 /pmc/articles/PMC9832202/ /pubmed/36626017 http://dx.doi.org/10.1186/s42649-022-00084-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Shin, Sumin
Hwang, Su-Kyeong
Mun, Ji Young
Changes of lysosome by L-serine in rotenone-treated hippocampal neurons
title Changes of lysosome by L-serine in rotenone-treated hippocampal neurons
title_full Changes of lysosome by L-serine in rotenone-treated hippocampal neurons
title_fullStr Changes of lysosome by L-serine in rotenone-treated hippocampal neurons
title_full_unstemmed Changes of lysosome by L-serine in rotenone-treated hippocampal neurons
title_short Changes of lysosome by L-serine in rotenone-treated hippocampal neurons
title_sort changes of lysosome by l-serine in rotenone-treated hippocampal neurons
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832202/
https://www.ncbi.nlm.nih.gov/pubmed/36626017
http://dx.doi.org/10.1186/s42649-022-00084-z
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