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Highly sensitive pork meat detection using copper(ii) tetraaza complex by electrochemical biosensor
Three copper(ii) tetraaza complexes [Cu(ii)LBr]Br (1a), [Cu(ii)L(CIO(4))](CIO(4)) (2a) and [Cu(ii)L](CIO(4))(2) (2b), where L = 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-7,14-diene were prepared and confirmed by FTIR, (1)HNMR and (13)CNMR. The binding interaction of complex (1a, 2a,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832347/ https://www.ncbi.nlm.nih.gov/pubmed/36712615 http://dx.doi.org/10.1039/d2ra05701h |
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author | Jilani, Noraisyah Abdul Kadir Zakariah, Emma Izzati Ariffin, Eda Yuhana Sapari, Suhaila Nokarajoo, Devika Yamin, Bohari Hasbullah, Siti Aishah |
author_facet | Jilani, Noraisyah Abdul Kadir Zakariah, Emma Izzati Ariffin, Eda Yuhana Sapari, Suhaila Nokarajoo, Devika Yamin, Bohari Hasbullah, Siti Aishah |
author_sort | Jilani, Noraisyah Abdul Kadir |
collection | PubMed |
description | Three copper(ii) tetraaza complexes [Cu(ii)LBr]Br (1a), [Cu(ii)L(CIO(4))](CIO(4)) (2a) and [Cu(ii)L](CIO(4))(2) (2b), where L = 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-7,14-diene were prepared and confirmed by FTIR, (1)HNMR and (13)CNMR. The binding interaction of complex (1a, 2a, 2b) with calf thymus DNA (CT-DNA) was investigated using UV-vis absorption, luminescence titrations, viscosity measurements and molecular docking. The findings suggested that complex 1a, 2a and 2b bind to DNA by electrostatic interaction, and the strengths of the interaction were arranged according to 2b > 1a > 2a. The differences in binding strengths were certainly caused by the complexes' dissimilar charges and counter anions. Complex 2b, with the biggest binding strength towards the DNA, was further applied in developing the porcine sensor. The developed sensor exhibits a broad linear dynamic range, low detection limit, good selectivity, and reproducibility. Analysis of real samples showed that the biosensor had excellent selectivity towards the pork meat compared to chicken and beef meat. |
format | Online Article Text |
id | pubmed-9832347 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-98323472023-01-26 Highly sensitive pork meat detection using copper(ii) tetraaza complex by electrochemical biosensor Jilani, Noraisyah Abdul Kadir Zakariah, Emma Izzati Ariffin, Eda Yuhana Sapari, Suhaila Nokarajoo, Devika Yamin, Bohari Hasbullah, Siti Aishah RSC Adv Chemistry Three copper(ii) tetraaza complexes [Cu(ii)LBr]Br (1a), [Cu(ii)L(CIO(4))](CIO(4)) (2a) and [Cu(ii)L](CIO(4))(2) (2b), where L = 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-7,14-diene were prepared and confirmed by FTIR, (1)HNMR and (13)CNMR. The binding interaction of complex (1a, 2a, 2b) with calf thymus DNA (CT-DNA) was investigated using UV-vis absorption, luminescence titrations, viscosity measurements and molecular docking. The findings suggested that complex 1a, 2a and 2b bind to DNA by electrostatic interaction, and the strengths of the interaction were arranged according to 2b > 1a > 2a. The differences in binding strengths were certainly caused by the complexes' dissimilar charges and counter anions. Complex 2b, with the biggest binding strength towards the DNA, was further applied in developing the porcine sensor. The developed sensor exhibits a broad linear dynamic range, low detection limit, good selectivity, and reproducibility. Analysis of real samples showed that the biosensor had excellent selectivity towards the pork meat compared to chicken and beef meat. The Royal Society of Chemistry 2023-01-11 /pmc/articles/PMC9832347/ /pubmed/36712615 http://dx.doi.org/10.1039/d2ra05701h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Jilani, Noraisyah Abdul Kadir Zakariah, Emma Izzati Ariffin, Eda Yuhana Sapari, Suhaila Nokarajoo, Devika Yamin, Bohari Hasbullah, Siti Aishah Highly sensitive pork meat detection using copper(ii) tetraaza complex by electrochemical biosensor |
title | Highly sensitive pork meat detection using copper(ii) tetraaza complex by electrochemical biosensor |
title_full | Highly sensitive pork meat detection using copper(ii) tetraaza complex by electrochemical biosensor |
title_fullStr | Highly sensitive pork meat detection using copper(ii) tetraaza complex by electrochemical biosensor |
title_full_unstemmed | Highly sensitive pork meat detection using copper(ii) tetraaza complex by electrochemical biosensor |
title_short | Highly sensitive pork meat detection using copper(ii) tetraaza complex by electrochemical biosensor |
title_sort | highly sensitive pork meat detection using copper(ii) tetraaza complex by electrochemical biosensor |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832347/ https://www.ncbi.nlm.nih.gov/pubmed/36712615 http://dx.doi.org/10.1039/d2ra05701h |
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