Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches

We present a novel genome-wide off-target prediction method named Extru-seq and compare it with cell-based (GUIDE-seq), in vitro (Digenome-seq), and in silico methods using promiscuous guide RNAs with large numbers of valid off-target sites. Extru-seq demonstrates a high validation rate and retentio...

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Detalles Bibliográficos
Autores principales: Kwon, Jeonghun, Kim, Minyoung, Hwang, Woochang, Jo, Anna, Hwang, Gue-Ho, Jung, Minhee, Kim, Un Gi, Cui, Gang, Kim, Heonseok, Eom, Joon-Ho, Hur, Junho K., Lee, Junwon, Kim, Youngho, Kim, Jin-soo, Bae, Sangsu, Lee, Jungjoon K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832775/
https://www.ncbi.nlm.nih.gov/pubmed/36627653
http://dx.doi.org/10.1186/s13059-022-02842-4

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