CoDe: a web-based tool for codon deoptimization

SUMMARY: We have developed a web-based tool, CoDe (Codon Deoptimization) that deoptimizes genetic sequences based on different codon usage bias, ultimately reducing expression of the corresponding protein. The tool could also deoptimize the sequence for a specific region and/or selected amino acid(s...

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Detalles Bibliográficos
Autores principales: Sharma, Divya, Baas, Tracey, Nogales, Aitor, Martinez-Sobrido, Luis, Gromiha, M Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Application Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832946/
https://www.ncbi.nlm.nih.gov/pubmed/36698765
http://dx.doi.org/10.1093/bioadv/vbac102
Descripción
Sumario:SUMMARY: We have developed a web-based tool, CoDe (Codon Deoptimization) that deoptimizes genetic sequences based on different codon usage bias, ultimately reducing expression of the corresponding protein. The tool could also deoptimize the sequence for a specific region and/or selected amino acid(s). Moreover, CoDe can highlight sites targeted by restriction enzymes in the wild-type and codon-deoptimized sequences. Importantly, our web-based tool has a user-friendly interface with flexible options to download results. AVAILABILITY AND IMPLEMENTATION: The web-based tool CoDe is freely available at https://web.iitm.ac.in/bioinfo2/codeop/landing_page.html. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics Advances online.

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