Cargando…

Cost-analysis of real time RT-PCR test performed for COVID-19 diagnosis at India’s national reference laboratory during the early stages of pandemic mitigation

Real-time reverse transcription polymerase chain reaction (rRT-PCR) is one of the most accurate and extensively used laboratory procedures for diagnosing COVID-19. This molecular test has high diagnostic accuracy (sensitivity and specificity) and is considered as the gold standard for COVID-19 diagn...

Descripción completa

Detalles Bibliográficos
Autores principales:	Minhas, Naveen, Gurav, Yogesh K., Sambhare, Susmit, Potdar, Varsha, Choudhary, Manohar Lal, Bhardwaj, Sumit Dutt, Abraham, Priya
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9833513/ https://www.ncbi.nlm.nih.gov/pubmed/36630456 http://dx.doi.org/10.1371/journal.pone.0277867

Ejemplares similares

Epidemiological characterization of COVID-19 – Pune, 2020-2021
por: Bhardwaj, Sumit D., et al.
Publicado: (2021)

Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR
por: Choudhary, Manohar L., et al.
Publicado: (2013)

Development of in vitro transcribed RNA as positive control for laboratory diagnosis of SARS-CoV-2 in India
por: Choudhary, Manohar Lal, et al.
Publicado: (2020)

Utility of a modified heat inactivation method for direct detection of SARS-CoV-2 by RT-qPCR in viral transport medium bypassing RNA extraction: A preliminary study
por: Alagarasu, Kalichami, et al.
Publicado: (2020)

Evaluation of RdRp & ORF-1b-nsp14-based real-time RT-PCR assays for confirmation of SARS-CoV-2 infection: An observational study
por: Alagarasu, K., et al.
Publicado: (2020)

Respiratory virus detection among the overseas returnees during the early phase of COVID-19 pandemic in India
por: Potdar, Varsha, et al.
Publicado: (2020)

Pro-inflammatory CXCL-10, TNF-α, IL-1β, and IL-6: biomarkers of SARS-CoV-2 infection
por: Tripathy, Anuradha S., et al.
Publicado: (2021)

Development of real-time RT-PCR for detection of human metapneumovirus and genetic analysis of circulating strains (2009-2011) in Pune, India
por: Choudhary, Manohar Lal, et al.
Publicado: (2013)

SARS-CoV-2 and Influenza Virus Co-Infection Cases Identified through ILI/SARI Sentinel Surveillance: A Pan-India Report
por: Aggarwal, Neeraj, et al.
Publicado: (2022)

Multisite surveillance for influenza and other respiratory viruses in India: 2016–2018
por: Chadha, Mandeep, et al.
Publicado: (2022)

MD simulation studies to investigate iso-energetic conformational behaviour of modified nucleosides m(2)G and m(2) (2)G present in tRNA
por: Bavi, Rohit S, et al.
Publicado: (2013)

Identification of Human Case of Avian Influenza A(H5N1) Infection, India
por: Potdar, Varsha, et al.
Publicado: (2022)

Idiosyncratic recognition of UUG/UUA codons by modified nucleoside 5-taurinomethyluridine, τm(5)U present at ‘wobble’ position in anticodon loop of tRNA(Leu): A molecular modeling approach
por: Kamble, Asmita S., et al.
Publicado: (2017)

Peripheral lymphocyte subset alteration in patients with COVID-19 having differential clinical manifestations
por: Tripathy, Anuradha S., et al.
Publicado: (2022)

SARS-CoV-2 seropositivity among non-medical frontline workers in Pune, Maharashtra, India
por: Deoshatwar, Avinash R., et al.
Publicado: (2022)

External quality assessment of COVID-19 real time reverse transcription PCR laboratories in India
por: Kaur, Harmanmeet, et al.
Publicado: (2022)

A case report of the enterovirus-D68 associated severe acute respiratory illness in a pediatric case from India
por: Bhardwaj, Sumit D., et al.
Publicado: (2019)

A retrospective analysis of respiratory virus transmission before and during the COVID-19 pandemic in Pune the western region of India
por: Bhardwaj, Sumit, et al.
Publicado: (2022)

Genetic characterization of SARS-CoV-2 & implications for epidemiology, diagnostics & vaccines in India
por: Abraham, Priya, et al.
Publicado: (2020)

Laboratory verification of an RT‐PCR assay for SARS‐CoV‐2
por: Wu, Yingping, et al.
Publicado: (2020)

Beyond Diagnostics: The role of a RT-PCR laboratory in a pandemic
por: Srikanth, P., et al.
Publicado: (2022)

Pandemic Influenza (H1N1) 2009 Is Associated with Severe Disease in India
por: Mishra, Akhilesh C., et al.
Publicado: (2010)

Full-genome sequences of the first two SARS-CoV-2 viruses from India
por: Yadav, Pragya D., et al.
Publicado: (2020)

Evaluation of droplet digital qRT-PCR (dd qRT-PCR) for quantification of SARS CoV-2 RNA in stool and urine specimens of COVID-19 patients
por: Shinde, Manohar, et al.
Publicado: (2023)

Correction to: Detection of SARS‑CoV‑2 variants in India from UK returnees
por: Potdar, Varsha, et al.
Publicado: (2021)

Detection of SARS-CoV-2 variants in India from UK returnees
por: Potdar, Varsha, et al.
Publicado: (2021)

Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions
por: Svingen, Terje, et al.
Publicado: (2015)

Longitudinal clinico-serological analysis of anti-nucleocapsid and anti-receptor binding domain of spike protein antibodies against SARS-CoV-2
por: Deshpande, Gururaj Rao, et al.
Publicado: (2021)

Evaluation of Candidate Reference Genes for Gene Expression Normalization in Brassica juncea Using Real Time Quantitative RT-PCR
por: Chandna, Ruby, et al.
Publicado: (2012)

Selection of optimal reference genes for normalization in quantitative RT-PCR
por: Chervoneva, Inna, et al.
Publicado: (2010)

Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples
por: Yu, Xuelian, et al.
Publicado: (2014)

Transmission electron microscopy imaging of SARS-CoV-2
por: Prasad, Sharda, et al.
Publicado: (2020)

Comparison of a laboratory-developed RT-PCR and the CDC RT-PCR protocol with rapid immunodiagnostic testing during the 2009 H1N1 influenza A pandemic
por: Liao, Robert Steven, et al.
Publicado: (2011)

Importance of real-time RT-PCR to supplement the laboratory diagnosis in the measles elimination program in China
por: Cui, Aili, et al.
Publicado: (2018)

P205 Development and inter-laboratory analysis of a quantitative influenza A RT-PCR
por: Luinstra, K., et al.
Publicado: (2009)

Laboratory Validation of a Real-Time RT-PCR Assay for the Detection of Jamestown Canyon Virus
por: Hughes, Holly R., et al.
Publicado: (2022)

Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)
por: Jeong, Jae-Kyo, et al.
Publicado: (2014)

Entry-inhibitory role of catechins against SARS-CoV-2 and its UK variant
por: Mhatre, Susmit, et al.
Publicado: (2021)

Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
por: Paolacci, Anna R, et al.
Publicado: (2009)

Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
por: Pinto, Filipe, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_krhg28gsfbph4mphttjb620f5b