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Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae)
The W chromosome of Lepidoptera is typically gene-poor, repeat-rich and composed of heterochromatin. Pioneering studies investigating this chromosome reported an abundance of mobile elements. However, the actual composition of the W chromosome varies greatly between species, as repeatedly demonstrat...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9834309/ https://www.ncbi.nlm.nih.gov/pubmed/36631492 http://dx.doi.org/10.1038/s41598-023-27757-3 |
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author | Hejníčková, Martina Dalíková, Martina Zrzavá, Magda Marec, František Lorite, Pedro Montiel, Eugenia E. |
author_facet | Hejníčková, Martina Dalíková, Martina Zrzavá, Magda Marec, František Lorite, Pedro Montiel, Eugenia E. |
author_sort | Hejníčková, Martina |
collection | PubMed |
description | The W chromosome of Lepidoptera is typically gene-poor, repeat-rich and composed of heterochromatin. Pioneering studies investigating this chromosome reported an abundance of mobile elements. However, the actual composition of the W chromosome varies greatly between species, as repeatedly demonstrated by comparative genomic hybridization (CGH) or fluorescence in situ hybridization (FISH). Here we present an analysis of repeats on the W chromosome in the willow beauty, Peribatodes rhomboidaria (Geometridae), a species in which CGH predicted an abundance of W-enriched or W-specific sequences. Indeed, comparative analysis of male and female genomes using RepeatExplorer identified ten putative W chromosome-enriched repeats, most of which are LTR or LINE mobile elements. We analysed the two most abundant: PRW LINE-like and PRW Bel-Pao. The results of FISH mapping and bioinformatic analysis confirmed their enrichment on the W chromosome, supporting the hypothesis that mobile elements are the driving force of W chromosome differentiation in Lepidoptera. As the W chromosome is highly underrepresented in chromosome-level genome assemblies of Lepidoptera, this recently introduced approach, combining bioinformatic comparative genome analysis with molecular cytogenetics, provides an elegant tool for studying this elusive and rapidly evolving part of the genome. |
format | Online Article Text |
id | pubmed-9834309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-98343092023-01-13 Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae) Hejníčková, Martina Dalíková, Martina Zrzavá, Magda Marec, František Lorite, Pedro Montiel, Eugenia E. Sci Rep Article The W chromosome of Lepidoptera is typically gene-poor, repeat-rich and composed of heterochromatin. Pioneering studies investigating this chromosome reported an abundance of mobile elements. However, the actual composition of the W chromosome varies greatly between species, as repeatedly demonstrated by comparative genomic hybridization (CGH) or fluorescence in situ hybridization (FISH). Here we present an analysis of repeats on the W chromosome in the willow beauty, Peribatodes rhomboidaria (Geometridae), a species in which CGH predicted an abundance of W-enriched or W-specific sequences. Indeed, comparative analysis of male and female genomes using RepeatExplorer identified ten putative W chromosome-enriched repeats, most of which are LTR or LINE mobile elements. We analysed the two most abundant: PRW LINE-like and PRW Bel-Pao. The results of FISH mapping and bioinformatic analysis confirmed their enrichment on the W chromosome, supporting the hypothesis that mobile elements are the driving force of W chromosome differentiation in Lepidoptera. As the W chromosome is highly underrepresented in chromosome-level genome assemblies of Lepidoptera, this recently introduced approach, combining bioinformatic comparative genome analysis with molecular cytogenetics, provides an elegant tool for studying this elusive and rapidly evolving part of the genome. Nature Publishing Group UK 2023-01-11 /pmc/articles/PMC9834309/ /pubmed/36631492 http://dx.doi.org/10.1038/s41598-023-27757-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hejníčková, Martina Dalíková, Martina Zrzavá, Magda Marec, František Lorite, Pedro Montiel, Eugenia E. Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae) |
title | Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes
rhomboidaria (Lepidoptera: Geometridae) |
title_full | Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes
rhomboidaria (Lepidoptera: Geometridae) |
title_fullStr | Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes
rhomboidaria (Lepidoptera: Geometridae) |
title_full_unstemmed | Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes
rhomboidaria (Lepidoptera: Geometridae) |
title_short | Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes
rhomboidaria (Lepidoptera: Geometridae) |
title_sort | accumulation of retrotransposons contributes to w chromosome differentiation in the willow beauty peribatodes
rhomboidaria (lepidoptera: geometridae) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9834309/ https://www.ncbi.nlm.nih.gov/pubmed/36631492 http://dx.doi.org/10.1038/s41598-023-27757-3 |
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