Engineering a Yellow Thermostable Fluorescent Protein by Rational Design

[Image: see text] Thermal green protein (TGP) is an extremely stable, highly soluble synthetic green fluorescent protein. The quantum yield of TGP is lower than the closest related natural fluorescent protein, monomeric Azami-Green. We improved the thermal recovery of TGP through the introduction of...

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Autores principales: Anderson, Matthew R., Padgett, Caitlin M., Dargatz, Cammi J., Nichols, Calysta R., Vittalam, Keerti R., DeVore, Natasha M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9835079/
https://www.ncbi.nlm.nih.gov/pubmed/36643458
http://dx.doi.org/10.1021/acsomega.2c05005
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author Anderson, Matthew R.
Padgett, Caitlin M.
Dargatz, Cammi J.
Nichols, Calysta R.
Vittalam, Keerti R.
DeVore, Natasha M.
author_facet Anderson, Matthew R.
Padgett, Caitlin M.
Dargatz, Cammi J.
Nichols, Calysta R.
Vittalam, Keerti R.
DeVore, Natasha M.
author_sort Anderson, Matthew R.
collection PubMed
description [Image: see text] Thermal green protein (TGP) is an extremely stable, highly soluble synthetic green fluorescent protein. The quantum yield of TGP is lower than the closest related natural fluorescent protein, monomeric Azami-Green. We improved the thermal recovery of TGP through the introduction of a chromophore mutation, Q66E. Furthermore, we developed a yellow thermal protein (YTP) via mutation of histidine 193 to tyrosine. Incorporation of Q66E into YTP (YTP-E) improved chemostability and pH stability. Both YTP and YTP-E have superior thermostability compared to TGP or TGP-E. These proteins offer a new option for green or yellow fluorescence under harsh chemical or thermal conditions.
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spelling pubmed-98350792023-01-13 Engineering a Yellow Thermostable Fluorescent Protein by Rational Design Anderson, Matthew R. Padgett, Caitlin M. Dargatz, Cammi J. Nichols, Calysta R. Vittalam, Keerti R. DeVore, Natasha M. ACS Omega [Image: see text] Thermal green protein (TGP) is an extremely stable, highly soluble synthetic green fluorescent protein. The quantum yield of TGP is lower than the closest related natural fluorescent protein, monomeric Azami-Green. We improved the thermal recovery of TGP through the introduction of a chromophore mutation, Q66E. Furthermore, we developed a yellow thermal protein (YTP) via mutation of histidine 193 to tyrosine. Incorporation of Q66E into YTP (YTP-E) improved chemostability and pH stability. Both YTP and YTP-E have superior thermostability compared to TGP or TGP-E. These proteins offer a new option for green or yellow fluorescence under harsh chemical or thermal conditions. American Chemical Society 2022-12-28 /pmc/articles/PMC9835079/ /pubmed/36643458 http://dx.doi.org/10.1021/acsomega.2c05005 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Anderson, Matthew R.
Padgett, Caitlin M.
Dargatz, Cammi J.
Nichols, Calysta R.
Vittalam, Keerti R.
DeVore, Natasha M.
Engineering a Yellow Thermostable Fluorescent Protein by Rational Design
title Engineering a Yellow Thermostable Fluorescent Protein by Rational Design
title_full Engineering a Yellow Thermostable Fluorescent Protein by Rational Design
title_fullStr Engineering a Yellow Thermostable Fluorescent Protein by Rational Design
title_full_unstemmed Engineering a Yellow Thermostable Fluorescent Protein by Rational Design
title_short Engineering a Yellow Thermostable Fluorescent Protein by Rational Design
title_sort engineering a yellow thermostable fluorescent protein by rational design
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9835079/
https://www.ncbi.nlm.nih.gov/pubmed/36643458
http://dx.doi.org/10.1021/acsomega.2c05005
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