Cargando…

Development of multiplex RT‐ddPCR assays for detection of SARS‐CoV‐2 and other common respiratory virus infections

BACKGROUND: Measures for mitigation of Coronavirus Disease 2019 (COVID‐19) were set to reduce the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2). SARS‐CoV‐2 and other respiratory viruses share similar transmission routes and some common clinical manifestations. Co‐circulation...

Descripción completa

Detalles Bibliográficos
Autores principales:	Leong, Nathaniel K. C., Gu, Haogao, Ng, Daisy Y. M., Chang, Lydia D. J., Krishnan, Pavithra, Cheng, Samuel S. M., Peiris, Malik, Poon, Leo L. M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2022
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9835441/ https://www.ncbi.nlm.nih.gov/pubmed/36517993 http://dx.doi.org/10.1111/irv.13084

Ejemplares similares

Novel RT-ddPCR Assays for determining the transcriptional profile of SARS-CoV-2
por: Telwatte, Sushama, et al.
Publicado: (2021)

HIV reservoir quantification using cross-subtype multiplex ddPCR
por: Cassidy, Noah A.J., et al.
Publicado: (2021)

ddPCRclust: an R package and Shiny app for automated analysis of multiplexed ddPCR data
por: Brink, Benedikt G, et al.
Publicado: (2018)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

Development of a new methylation‐based fetal fraction estimation assay using multiplex ddPCR
por: Ioannides, Marios, et al.
Publicado: (2019)

Multiplex ddPCR: A Promising Diagnostic Assay for Early Detection and Drug Monitoring in Bovine Theileriosis
por: Murthy, Shweta, et al.
Publicado: (2023)

Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution
por: Van Poelvoorde, Laura A. E., et al.
Publicado: (2021)

Maximizing viral detection with SIV droplet digital PCR (ddPCR) assays
por: Long, Samuel, et al.
Publicado: (2020)

Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts
por: Telwatte, Sushama, et al.
Publicado: (2022)

Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater
por: Ciesielski, Mark, et al.
Publicado: (2021)

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
por: Long, Samuel, et al.
Publicado: (2021)

Novel RT-ddPCR assays for simultaneous quantification of multiple noncoding and coding regions of SARS-CoV-2 RNA
por: Telwatte, Sushama, et al.
Publicado: (2021)

Somatic Mutation Allelic Ratio Test Using ddPCR (SMART-ddPCR): An Accurate Method for Assessment of Preferential Allelic Imbalance in Tumor DNA
por: de Smith, Adam J., et al.
Publicado: (2015)

Quantification of the mobility potential of antibiotic resistance genes through multiplexed ddPCR linkage analysis
por: de la Cruz Barron, Magali, et al.
Publicado: (2023)

Development of a one-step RT-ddPCR method to determine the expression and potency of AAV vectors
por: Clarner, Pete, et al.
Publicado: (2021)

Droplet digital PCR (ddPCR) for the detection and quantification of Ureaplasma spp.
por: Huang, Yanfang, et al.
Publicado: (2021)

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets
por: Liu, Xinjin, et al.
Publicado: (2020)

Development and optimization of a simian immunodeficiency virus (SIV) droplet digital PCR (ddPCR) assay
por: Long, Samuel, et al.
Publicado: (2020)

Using ddPCR to assess the DNA yield of FFPE samples
por: Lu, X.J. David, et al.
Publicado: (2018)

Rapid, precise quantification of large DNA excisions and inversions by ddPCR
por: Watry, Hannah L., et al.
Publicado: (2020)

Respiratory Adenovirus Quantification with a Droplet Digital Polymerase Chain Reaction (ddPCR) Assay
por: Abdullah, Omar, et al.
Publicado: (2023)

Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion (Neophoca cinerea) and other mammals
por: Meza Cerda, María-Ignacia, et al.
Publicado: (2020)

Rapid and accurate identification of SARS-CoV-2 Omicron variants using droplet digital PCR (RT-ddPCR)
por: Mills, Margaret G., et al.
Publicado: (2022)

A droplet digital PCR (ddPCR) assay to detect Helicoverpa armigera (Lepidoptera: Noctuidae) in bulk trap samples
por: Zink, Frida A., et al.
Publicado: (2017)

Human herpesvirus multiplex ddPCR detection in brain tissue from low- and high-grade astrocytoma cases and controls
por: Lin, Cheng-Te Major, et al.
Publicado: (2016)

Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics
por: Rose, John C., et al.
Publicado: (2017)

Direct comparison of RT-ddPCR and targeted amplicon sequencing for SARS-CoV-2 mutation monitoring in wastewater
por: Lou, Esther G., et al.
Publicado: (2022)

Selection of reference genes for expression analysis of plant-derived microRNAs in Plutella xylostella using qRT-PCR and ddPCR
por: Zhang, Lingling, et al.
Publicado: (2019)

Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR)
por: He, Zhiyong, et al.
Publicado: (2023)

Standardization of the liquid biopsy for pediatric diffuse midline glioma using ddPCR
por: Li, Daphne, et al.
Publicado: (2021)

A custom ddPCR method for the detection of copy number variations in the nebulin triplicate region
por: Sagath, Lydia, et al.
Publicado: (2022)

Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue
por: Barney, Rachael E., et al.
Publicado: (2023)

Multiplexed ddPCR-amplicon sequencing reveals isolated Plasmodium falciparum populations amenable to local elimination in Zanzibar, Tanzania
por: Holzschuh, Aurel, et al.
Publicado: (2023)

The Effectiveness of ddPCR for Detection of Point Mutations in Poor-Quality Saliva Samples
por: Riedlova, Petra, et al.
Publicado: (2022)

Assay Optimization Can Equalize the Sensitivity of Real-Time PCR with ddPCR for Detection of Helicoverpa armigera (Lepidoptera: Noctuidae) in Bulk Samples
por: Oliveira, Thayssa M. R., et al.
Publicado: (2021)

Detection of Plasma EGFR Mutations in NSCLC Patients with a Validated ddPCR Lung cfDNA Assay
por: Guo, Qiao-mei, et al.
Publicado: (2019)

Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples
por: Pomari, Elena, et al.
Publicado: (2023)

Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)
por: Koepfli, Cristian, et al.
Publicado: (2016)

A qPCR Method for AAV Genome Titer with ddPCR-Level of Accuracy and Precision
por: Wang, Yu, et al.
Publicado: (2020)

Ultrasensitive detection and quantification of viral nucleic acids with Raindance droplet digital PCR (ddPCR)
por: Long, Samuel, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_u92bg9qo5cr3ebq08lshat6dpj