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Artificial Synapse: Spatiotemporal Heterogeneities in Dopamine Electrochemistry at a Carbon Fiber Ultramicroelectrode

[Image: see text] An artificial synapse is developed that mimics ultramicroelectrode (UME) amperometric detection of single cell exocytosis. It comprises the nanopipette of a scanning ion conductance microscope (SICM), which delivers rapid pulses of neurotransmitter (dopamine) locally and on demand...

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Detalles Bibliográficos
Autores principales: Chen, Baoping, Perry, David, Teahan, James, McPherson, Ian J., Edmondson, James, Kang, Minkyung, Valavanis, Dimitrios, Frenguelli, Bruno G., Unwin, Patrick R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836071/
https://www.ncbi.nlm.nih.gov/pubmed/36785735
http://dx.doi.org/10.1021/acsmeasuresciau.1c00006
Descripción
Sumario:[Image: see text] An artificial synapse is developed that mimics ultramicroelectrode (UME) amperometric detection of single cell exocytosis. It comprises the nanopipette of a scanning ion conductance microscope (SICM), which delivers rapid pulses of neurotransmitter (dopamine) locally and on demand at >1000 defined locations of a carbon fiber (CF) UME in each experiment. Analysis of the resulting UME current-space-time data reveals spatiotemporal heterogeneous electrode activity on the nanoscale and submillisecond time scale for dopamine electrooxidation at typical UME detection potentials. Through complementary surface charge mapping and finite element method (FEM) simulations, these previously unseen variations in electrochemical activity are related to heterogeneities in the surface chemistry of the CF UME.