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Evaluation of ABCA1 gene polymorphism as a prognostic index of fibrosis progression in NAFLD patients
INTRODUCTION: It had been evident that non‐alcoholic fatty liver disease (NAFLD) is the new era epidemic. Despite emergence of many drugs on the pipeline that considered candidates to cure NAFLD/NASH, the critical need for defining the cohort liable to fibrosis progression is yet unmet. AIM: Evaluat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836255/ https://www.ncbi.nlm.nih.gov/pubmed/36444680 http://dx.doi.org/10.1002/edm2.394 |
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author | Ghanem, Samar E. Elsabaawy, Maha M. Abdelkareem, Mervat M. Helal, Marwa L. Othman, Warda Elsayed, Mahitab Elsabaawy, Dalia M. El Fert, Ashraf |
author_facet | Ghanem, Samar E. Elsabaawy, Maha M. Abdelkareem, Mervat M. Helal, Marwa L. Othman, Warda Elsayed, Mahitab Elsabaawy, Dalia M. El Fert, Ashraf |
author_sort | Ghanem, Samar E. |
collection | PubMed |
description | INTRODUCTION: It had been evident that non‐alcoholic fatty liver disease (NAFLD) is the new era epidemic. Despite emergence of many drugs on the pipeline that considered candidates to cure NAFLD/NASH, the critical need for defining the cohort liable to fibrosis progression is yet unmet. AIM: Evaluate ABCA1 (rs1800977) genotyping as a noninvasive predictor of liver fibrosis severity. MATERIALS AND METHODS: This study included 118 liver biopsy‐proven NAFLD‐patients. According to Metavir‐fibrosis‐staging, cases were divided to early fibrosis (74 cases), and 44 cases with significant fibrosis (>F2), added to 49 healthy control subjects. All patients were subjected to liver function tests, lipids profile, triglyceride TG index, and hepatic steatosis index (HSI) and real‐time PCR ABCA1 SNP (rs1800977). RESULTS: Significant differences in transaminases (p > .05), albumin (p < .009), cholesterol (p0.03), low density lipoproteins (LDL) (0.006), triglycerides (p < .001), HSI (p < .001), FIB4 (p < .001) and APRI (p < .001) were reported in those with significant than early fibrosis and control groups. CC was the most prevalent in significant (36.4%) than early fibrosis (13.5%) and control groups (8.2%), with prevalence of C allele in significant fibrosis (p ≤ .003). Univariate analysis revealed that DM (p ≤ .001), TG index (p ≤ .022), cholesterol (p ≤ .03), HSI (p ≤ .006), LDL (p ≤ .02), HDL (p ≤ .01), APRI (p ≤ .02) and CC genotype (p ≤ .005) were the main factors affecting fibrosis progression in NAFLD. However multivariate analysis proved only the role of HSI (p ≤ .005), LDL (p ≤ .02), HDL (p ≤ .003) and CC genotype (p ≤ .03) in predicting fibrosis severity. CONCLUSION: Dyslipidemias, hepatic steatosis index and ABCA1 (rs1800977) gene polymorphism CC genotype; were the only independent predictors of advanced fibrosis in NAFLD‐patients. |
format | Online Article Text |
id | pubmed-9836255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98362552023-01-18 Evaluation of ABCA1 gene polymorphism as a prognostic index of fibrosis progression in NAFLD patients Ghanem, Samar E. Elsabaawy, Maha M. Abdelkareem, Mervat M. Helal, Marwa L. Othman, Warda Elsayed, Mahitab Elsabaawy, Dalia M. El Fert, Ashraf Endocrinol Diabetes Metab Research Articles INTRODUCTION: It had been evident that non‐alcoholic fatty liver disease (NAFLD) is the new era epidemic. Despite emergence of many drugs on the pipeline that considered candidates to cure NAFLD/NASH, the critical need for defining the cohort liable to fibrosis progression is yet unmet. AIM: Evaluate ABCA1 (rs1800977) genotyping as a noninvasive predictor of liver fibrosis severity. MATERIALS AND METHODS: This study included 118 liver biopsy‐proven NAFLD‐patients. According to Metavir‐fibrosis‐staging, cases were divided to early fibrosis (74 cases), and 44 cases with significant fibrosis (>F2), added to 49 healthy control subjects. All patients were subjected to liver function tests, lipids profile, triglyceride TG index, and hepatic steatosis index (HSI) and real‐time PCR ABCA1 SNP (rs1800977). RESULTS: Significant differences in transaminases (p > .05), albumin (p < .009), cholesterol (p0.03), low density lipoproteins (LDL) (0.006), triglycerides (p < .001), HSI (p < .001), FIB4 (p < .001) and APRI (p < .001) were reported in those with significant than early fibrosis and control groups. CC was the most prevalent in significant (36.4%) than early fibrosis (13.5%) and control groups (8.2%), with prevalence of C allele in significant fibrosis (p ≤ .003). Univariate analysis revealed that DM (p ≤ .001), TG index (p ≤ .022), cholesterol (p ≤ .03), HSI (p ≤ .006), LDL (p ≤ .02), HDL (p ≤ .01), APRI (p ≤ .02) and CC genotype (p ≤ .005) were the main factors affecting fibrosis progression in NAFLD. However multivariate analysis proved only the role of HSI (p ≤ .005), LDL (p ≤ .02), HDL (p ≤ .003) and CC genotype (p ≤ .03) in predicting fibrosis severity. CONCLUSION: Dyslipidemias, hepatic steatosis index and ABCA1 (rs1800977) gene polymorphism CC genotype; were the only independent predictors of advanced fibrosis in NAFLD‐patients. John Wiley and Sons Inc. 2022-11-29 /pmc/articles/PMC9836255/ /pubmed/36444680 http://dx.doi.org/10.1002/edm2.394 Text en © 2022 The Authors. Endocrinology, Diabetes & Metabolism published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Ghanem, Samar E. Elsabaawy, Maha M. Abdelkareem, Mervat M. Helal, Marwa L. Othman, Warda Elsayed, Mahitab Elsabaawy, Dalia M. El Fert, Ashraf Evaluation of ABCA1 gene polymorphism as a prognostic index of fibrosis progression in NAFLD patients |
title | Evaluation of ABCA1 gene polymorphism as a prognostic index of fibrosis progression in NAFLD patients |
title_full | Evaluation of ABCA1 gene polymorphism as a prognostic index of fibrosis progression in NAFLD patients |
title_fullStr | Evaluation of ABCA1 gene polymorphism as a prognostic index of fibrosis progression in NAFLD patients |
title_full_unstemmed | Evaluation of ABCA1 gene polymorphism as a prognostic index of fibrosis progression in NAFLD patients |
title_short | Evaluation of ABCA1 gene polymorphism as a prognostic index of fibrosis progression in NAFLD patients |
title_sort | evaluation of abca1 gene polymorphism as a prognostic index of fibrosis progression in nafld patients |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836255/ https://www.ncbi.nlm.nih.gov/pubmed/36444680 http://dx.doi.org/10.1002/edm2.394 |
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