Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification–based assay for GBS screening during pregnancy

Timely and accurate detection of Group B Streptococcus (GBS) carriage in pregnant women allows for targeted peripartum prophylaxis. Replacing culture-based screening by molecular biology assays enables faster results obtention, better targeted antibiotic prophylaxis, and reduces the laboratory workl...

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Autores principales: Tittel-Elmer, Mireille, de Tejada, Begoña Martinez, Renzi, Gesuele, Schrenzel, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836968/
https://www.ncbi.nlm.nih.gov/pubmed/36449147
http://dx.doi.org/10.1007/s10096-022-04534-0
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author Tittel-Elmer, Mireille
de Tejada, Begoña Martinez
Renzi, Gesuele
Schrenzel, Jacques
author_facet Tittel-Elmer, Mireille
de Tejada, Begoña Martinez
Renzi, Gesuele
Schrenzel, Jacques
author_sort Tittel-Elmer, Mireille
collection PubMed
description Timely and accurate detection of Group B Streptococcus (GBS) carriage in pregnant women allows for targeted peripartum prophylaxis. Replacing culture-based screening by molecular biology assays enables faster results obtention, better targeted antibiotic prophylaxis, and reduces the laboratory workload. Here, we present a comparative analysis between a Loop Mediated Isothermal Amplification assay (HiberGene GBS kit) and culture (gold-standard). The HiberGene GBS kit showed a sensitivity of 97.9% and a specificity of 96.8% compared with culture. The limit of detection was estimated at 10(3) cfu/ml and results were obtained within 30 min. HiberGene GBS assay can be used for peripartum GBS screening and targeted antibiotic prophylaxis provided sample processing can be swiftly performed around the clock.
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spelling pubmed-98369682023-01-14 Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification–based assay for GBS screening during pregnancy Tittel-Elmer, Mireille de Tejada, Begoña Martinez Renzi, Gesuele Schrenzel, Jacques Eur J Clin Microbiol Infect Dis Brief Report Timely and accurate detection of Group B Streptococcus (GBS) carriage in pregnant women allows for targeted peripartum prophylaxis. Replacing culture-based screening by molecular biology assays enables faster results obtention, better targeted antibiotic prophylaxis, and reduces the laboratory workload. Here, we present a comparative analysis between a Loop Mediated Isothermal Amplification assay (HiberGene GBS kit) and culture (gold-standard). The HiberGene GBS kit showed a sensitivity of 97.9% and a specificity of 96.8% compared with culture. The limit of detection was estimated at 10(3) cfu/ml and results were obtained within 30 min. HiberGene GBS assay can be used for peripartum GBS screening and targeted antibiotic prophylaxis provided sample processing can be swiftly performed around the clock. Springer Berlin Heidelberg 2022-11-30 2023 /pmc/articles/PMC9836968/ /pubmed/36449147 http://dx.doi.org/10.1007/s10096-022-04534-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Report
Tittel-Elmer, Mireille
de Tejada, Begoña Martinez
Renzi, Gesuele
Schrenzel, Jacques
Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification–based assay for GBS screening during pregnancy
title Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification–based assay for GBS screening during pregnancy
title_full Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification–based assay for GBS screening during pregnancy
title_fullStr Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification–based assay for GBS screening during pregnancy
title_full_unstemmed Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification–based assay for GBS screening during pregnancy
title_short Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification–based assay for GBS screening during pregnancy
title_sort performance of the hibergene group b streptococcus kit, a loop-mediated isothermal amplification–based assay for gbs screening during pregnancy
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836968/
https://www.ncbi.nlm.nih.gov/pubmed/36449147
http://dx.doi.org/10.1007/s10096-022-04534-0
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