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Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates

There is a continuing need for biosensors that can be used in the diagnosis of COVID-19 infection and to measure a subject’s immune response to the virus itself (SARS-CoV-2). In this study, grating-coupled fluorescent plasmonic (GC-FP)-based detection of SARS-CoV-2 antigens was coupled with antibody...

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Detalles Bibliográficos
Autores principales: Taubner, Benjamin, Gibbons, Ashley, Cady, Nathaniel C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837026/
https://www.ncbi.nlm.nih.gov/pubmed/35983838
http://dx.doi.org/10.1177/15353702221113860
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author Taubner, Benjamin
Gibbons, Ashley
Cady, Nathaniel C
author_facet Taubner, Benjamin
Gibbons, Ashley
Cady, Nathaniel C
author_sort Taubner, Benjamin
collection PubMed
description There is a continuing need for biosensors that can be used in the diagnosis of COVID-19 infection and to measure a subject’s immune response to the virus itself (SARS-CoV-2). In this study, grating-coupled fluorescent plasmonic (GC-FP)-based detection of SARS-CoV-2 antigens was coupled with antibody detection to yield a dual-mode detection assay. Pairs of capture and detection antibodies were screened for direct detection of the SARS-CoV-2 nucleocapsid (Nuc) antigen, which were then combined with an existing GC-FP antibody detection assay. Nuc could be detected as low as 1 µg/mL concentrations, while antibodies were detectable to 50 ng/mL. The dual detection assay was tested by adding purified Nuc antigen to serum from a polymerase chain reaction (PCR)-positive COVID-19-infected individual. Using this sample, co-detection of Nuc antigen and anti-spike protein antibodies was successfully performed on a single GC-FP chip. Total assay time was 1 h, making this the first known example of rapid dual antibody and antigen detection on the same biosensor chip.
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spelling pubmed-98370262023-01-13 Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates Taubner, Benjamin Gibbons, Ashley Cady, Nathaniel C Exp Biol Med (Maywood) Original Research There is a continuing need for biosensors that can be used in the diagnosis of COVID-19 infection and to measure a subject’s immune response to the virus itself (SARS-CoV-2). In this study, grating-coupled fluorescent plasmonic (GC-FP)-based detection of SARS-CoV-2 antigens was coupled with antibody detection to yield a dual-mode detection assay. Pairs of capture and detection antibodies were screened for direct detection of the SARS-CoV-2 nucleocapsid (Nuc) antigen, which were then combined with an existing GC-FP antibody detection assay. Nuc could be detected as low as 1 µg/mL concentrations, while antibodies were detectable to 50 ng/mL. The dual detection assay was tested by adding purified Nuc antigen to serum from a polymerase chain reaction (PCR)-positive COVID-19-infected individual. Using this sample, co-detection of Nuc antigen and anti-spike protein antibodies was successfully performed on a single GC-FP chip. Total assay time was 1 h, making this the first known example of rapid dual antibody and antigen detection on the same biosensor chip. SAGE Publications 2022-08-19 2022-12 /pmc/articles/PMC9837026/ /pubmed/35983838 http://dx.doi.org/10.1177/15353702221113860 Text en © Experimental Biology and Medicine 2022
spellingShingle Original Research
Taubner, Benjamin
Gibbons, Ashley
Cady, Nathaniel C
Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates
title Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates
title_full Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates
title_fullStr Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates
title_full_unstemmed Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates
title_short Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates
title_sort dual detection of covid-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837026/
https://www.ncbi.nlm.nih.gov/pubmed/35983838
http://dx.doi.org/10.1177/15353702221113860
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