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Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates
There is a continuing need for biosensors that can be used in the diagnosis of COVID-19 infection and to measure a subject’s immune response to the virus itself (SARS-CoV-2). In this study, grating-coupled fluorescent plasmonic (GC-FP)-based detection of SARS-CoV-2 antigens was coupled with antibody...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837026/ https://www.ncbi.nlm.nih.gov/pubmed/35983838 http://dx.doi.org/10.1177/15353702221113860 |
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author | Taubner, Benjamin Gibbons, Ashley Cady, Nathaniel C |
author_facet | Taubner, Benjamin Gibbons, Ashley Cady, Nathaniel C |
author_sort | Taubner, Benjamin |
collection | PubMed |
description | There is a continuing need for biosensors that can be used in the diagnosis of COVID-19 infection and to measure a subject’s immune response to the virus itself (SARS-CoV-2). In this study, grating-coupled fluorescent plasmonic (GC-FP)-based detection of SARS-CoV-2 antigens was coupled with antibody detection to yield a dual-mode detection assay. Pairs of capture and detection antibodies were screened for direct detection of the SARS-CoV-2 nucleocapsid (Nuc) antigen, which were then combined with an existing GC-FP antibody detection assay. Nuc could be detected as low as 1 µg/mL concentrations, while antibodies were detectable to 50 ng/mL. The dual detection assay was tested by adding purified Nuc antigen to serum from a polymerase chain reaction (PCR)-positive COVID-19-infected individual. Using this sample, co-detection of Nuc antigen and anti-spike protein antibodies was successfully performed on a single GC-FP chip. Total assay time was 1 h, making this the first known example of rapid dual antibody and antigen detection on the same biosensor chip. |
format | Online Article Text |
id | pubmed-9837026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-98370262023-01-13 Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates Taubner, Benjamin Gibbons, Ashley Cady, Nathaniel C Exp Biol Med (Maywood) Original Research There is a continuing need for biosensors that can be used in the diagnosis of COVID-19 infection and to measure a subject’s immune response to the virus itself (SARS-CoV-2). In this study, grating-coupled fluorescent plasmonic (GC-FP)-based detection of SARS-CoV-2 antigens was coupled with antibody detection to yield a dual-mode detection assay. Pairs of capture and detection antibodies were screened for direct detection of the SARS-CoV-2 nucleocapsid (Nuc) antigen, which were then combined with an existing GC-FP antibody detection assay. Nuc could be detected as low as 1 µg/mL concentrations, while antibodies were detectable to 50 ng/mL. The dual detection assay was tested by adding purified Nuc antigen to serum from a polymerase chain reaction (PCR)-positive COVID-19-infected individual. Using this sample, co-detection of Nuc antigen and anti-spike protein antibodies was successfully performed on a single GC-FP chip. Total assay time was 1 h, making this the first known example of rapid dual antibody and antigen detection on the same biosensor chip. SAGE Publications 2022-08-19 2022-12 /pmc/articles/PMC9837026/ /pubmed/35983838 http://dx.doi.org/10.1177/15353702221113860 Text en © Experimental Biology and Medicine 2022 |
spellingShingle | Original Research Taubner, Benjamin Gibbons, Ashley Cady, Nathaniel C Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates |
title | Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates |
title_full | Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates |
title_fullStr | Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates |
title_full_unstemmed | Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates |
title_short | Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates |
title_sort | dual detection of covid-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837026/ https://www.ncbi.nlm.nih.gov/pubmed/35983838 http://dx.doi.org/10.1177/15353702221113860 |
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