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Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein
Acrolein (AC) is one of the most toxic environmental pollutants, often associated with incomplete combustion of petrol, wood, and plastic, oil frying, and tobacco smoking, that causes oxidative damage to DNA and mitochondria. Considering that little is known about the protective effects of whey prot...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837534/ https://www.ncbi.nlm.nih.gov/pubmed/36226819 http://dx.doi.org/10.2478/aiht-2022-73-3640 |
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author | Aydın, Birsen Oğuz, Ali Şekeroğlu, Vedat Atlı Şekeroğlu, Zülal |
author_facet | Aydın, Birsen Oğuz, Ali Şekeroğlu, Vedat Atlı Şekeroğlu, Zülal |
author_sort | Aydın, Birsen |
collection | PubMed |
description | Acrolein (AC) is one of the most toxic environmental pollutants, often associated with incomplete combustion of petrol, wood, and plastic, oil frying, and tobacco smoking, that causes oxidative damage to DNA and mitochondria. Considering that little is known about the protective effects of whey protein (WP) against AC-induced liver toxicity, the aim of our study was to learn more about them in respect to liver mitochondrial oxidative stress, respiratory enzymes, Krebs cycle enzymes, and adenosine triphosphate (ATP). To do that, we treated Sprague Dawley rats with daily doses of AC alone (5 mg/kg bw in 0.9 % NaCl solution), WP alone (200 mg/kg bw, in 0.9 % NaCl solution), or their combination by oral gavage for six days a week over 30 days. As expected, the AC group showed a drop in glutathione levels and antioxidant, transport chain, and tricarboxylic acid cycle enzyme activities and a significant rise in mitochondrial lipid peroxidation and protein carbonyl levels. Co-treatment with WP mitigated oxidative stress and improved enzyme activities. Judging by the measured parameters, WP reduced AC toxicity by improving bioenergetic mechanisms and eliminating oxidative stress. |
format | Online Article Text |
id | pubmed-9837534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-98375342023-01-23 Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein Aydın, Birsen Oğuz, Ali Şekeroğlu, Vedat Atlı Şekeroğlu, Zülal Arh Hig Rada Toksikol Original Article Acrolein (AC) is one of the most toxic environmental pollutants, often associated with incomplete combustion of petrol, wood, and plastic, oil frying, and tobacco smoking, that causes oxidative damage to DNA and mitochondria. Considering that little is known about the protective effects of whey protein (WP) against AC-induced liver toxicity, the aim of our study was to learn more about them in respect to liver mitochondrial oxidative stress, respiratory enzymes, Krebs cycle enzymes, and adenosine triphosphate (ATP). To do that, we treated Sprague Dawley rats with daily doses of AC alone (5 mg/kg bw in 0.9 % NaCl solution), WP alone (200 mg/kg bw, in 0.9 % NaCl solution), or their combination by oral gavage for six days a week over 30 days. As expected, the AC group showed a drop in glutathione levels and antioxidant, transport chain, and tricarboxylic acid cycle enzyme activities and a significant rise in mitochondrial lipid peroxidation and protein carbonyl levels. Co-treatment with WP mitigated oxidative stress and improved enzyme activities. Judging by the measured parameters, WP reduced AC toxicity by improving bioenergetic mechanisms and eliminating oxidative stress. Sciendo 2022-09-30 /pmc/articles/PMC9837534/ /pubmed/36226819 http://dx.doi.org/10.2478/aiht-2022-73-3640 Text en © 2022 Birsen Aydın, Ali Oğuz, Vedat Şekeroğlu, and Zülal Atlı Şekeroğlu, published by Sciendo https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Original Article Aydın, Birsen Oğuz, Ali Şekeroğlu, Vedat Atlı Şekeroğlu, Zülal Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein |
title | Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein |
title_full | Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein |
title_fullStr | Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein |
title_full_unstemmed | Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein |
title_short | Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein |
title_sort | whey protein protects liver mitochondrial function against oxidative stress in rats exposed to acrolein |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837534/ https://www.ncbi.nlm.nih.gov/pubmed/36226819 http://dx.doi.org/10.2478/aiht-2022-73-3640 |
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