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Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein

Acrolein (AC) is one of the most toxic environmental pollutants, often associated with incomplete combustion of petrol, wood, and plastic, oil frying, and tobacco smoking, that causes oxidative damage to DNA and mitochondria. Considering that little is known about the protective effects of whey prot...

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Autores principales: Aydın, Birsen, Oğuz, Ali, Şekeroğlu, Vedat, Atlı Şekeroğlu, Zülal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837534/
https://www.ncbi.nlm.nih.gov/pubmed/36226819
http://dx.doi.org/10.2478/aiht-2022-73-3640
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author Aydın, Birsen
Oğuz, Ali
Şekeroğlu, Vedat
Atlı Şekeroğlu, Zülal
author_facet Aydın, Birsen
Oğuz, Ali
Şekeroğlu, Vedat
Atlı Şekeroğlu, Zülal
author_sort Aydın, Birsen
collection PubMed
description Acrolein (AC) is one of the most toxic environmental pollutants, often associated with incomplete combustion of petrol, wood, and plastic, oil frying, and tobacco smoking, that causes oxidative damage to DNA and mitochondria. Considering that little is known about the protective effects of whey protein (WP) against AC-induced liver toxicity, the aim of our study was to learn more about them in respect to liver mitochondrial oxidative stress, respiratory enzymes, Krebs cycle enzymes, and adenosine triphosphate (ATP). To do that, we treated Sprague Dawley rats with daily doses of AC alone (5 mg/kg bw in 0.9 % NaCl solution), WP alone (200 mg/kg bw, in 0.9 % NaCl solution), or their combination by oral gavage for six days a week over 30 days. As expected, the AC group showed a drop in glutathione levels and antioxidant, transport chain, and tricarboxylic acid cycle enzyme activities and a significant rise in mitochondrial lipid peroxidation and protein carbonyl levels. Co-treatment with WP mitigated oxidative stress and improved enzyme activities. Judging by the measured parameters, WP reduced AC toxicity by improving bioenergetic mechanisms and eliminating oxidative stress.
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spelling pubmed-98375342023-01-23 Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein Aydın, Birsen Oğuz, Ali Şekeroğlu, Vedat Atlı Şekeroğlu, Zülal Arh Hig Rada Toksikol Original Article Acrolein (AC) is one of the most toxic environmental pollutants, often associated with incomplete combustion of petrol, wood, and plastic, oil frying, and tobacco smoking, that causes oxidative damage to DNA and mitochondria. Considering that little is known about the protective effects of whey protein (WP) against AC-induced liver toxicity, the aim of our study was to learn more about them in respect to liver mitochondrial oxidative stress, respiratory enzymes, Krebs cycle enzymes, and adenosine triphosphate (ATP). To do that, we treated Sprague Dawley rats with daily doses of AC alone (5 mg/kg bw in 0.9 % NaCl solution), WP alone (200 mg/kg bw, in 0.9 % NaCl solution), or their combination by oral gavage for six days a week over 30 days. As expected, the AC group showed a drop in glutathione levels and antioxidant, transport chain, and tricarboxylic acid cycle enzyme activities and a significant rise in mitochondrial lipid peroxidation and protein carbonyl levels. Co-treatment with WP mitigated oxidative stress and improved enzyme activities. Judging by the measured parameters, WP reduced AC toxicity by improving bioenergetic mechanisms and eliminating oxidative stress. Sciendo 2022-09-30 /pmc/articles/PMC9837534/ /pubmed/36226819 http://dx.doi.org/10.2478/aiht-2022-73-3640 Text en © 2022 Birsen Aydın, Ali Oğuz, Vedat Şekeroğlu, and Zülal Atlı Şekeroğlu, published by Sciendo https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Original Article
Aydın, Birsen
Oğuz, Ali
Şekeroğlu, Vedat
Atlı Şekeroğlu, Zülal
Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein
title Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein
title_full Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein
title_fullStr Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein
title_full_unstemmed Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein
title_short Whey Protein Protects Liver Mitochondrial Function Against Oxidative Stress in Rats Exposed to Acrolein
title_sort whey protein protects liver mitochondrial function against oxidative stress in rats exposed to acrolein
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837534/
https://www.ncbi.nlm.nih.gov/pubmed/36226819
http://dx.doi.org/10.2478/aiht-2022-73-3640
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