Cargando…

Ring finger protein 128 promotes, rather than inhibits, colorectal cancer progression by regulating the Hippo signaling pathway

BACKGROUND: Colorectal cancer is a common malignancy of the gastrointestinal tract, and its incidence and mortality rates have increased in recent years. RNF128 is an E3 ubiquitin ligase that plays an important role as a suppressor gene or oncogene in various cancers, but its mechanism in colorectal...

Descripción completa

Detalles Bibliográficos
Autores principales: Ning, Shili, Chen, Yuzhuo, Wang, Guangzhi, Liu, Yongtai, Yang, Yingchi, Zhang, Zhongtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837830/
https://www.ncbi.nlm.nih.gov/pubmed/36644633
http://dx.doi.org/10.3389/fonc.2022.1031160
_version_ 1784869157248958464
author Ning, Shili
Chen, Yuzhuo
Wang, Guangzhi
Liu, Yongtai
Yang, Yingchi
Zhang, Zhongtao
author_facet Ning, Shili
Chen, Yuzhuo
Wang, Guangzhi
Liu, Yongtai
Yang, Yingchi
Zhang, Zhongtao
author_sort Ning, Shili
collection PubMed
description BACKGROUND: Colorectal cancer is a common malignancy of the gastrointestinal tract, and its incidence and mortality rates have increased in recent years. RNF128 is an E3 ubiquitin ligase that plays an important role as a suppressor gene or oncogene in various cancers, but its mechanism in colorectal cancer is not yet clear. The aim of this study was to investigate the role and mechanism of RNF128 in colorectal cancer. METHODS: The expression of RNF128 in colorectal cancer tissues was assessed by immunohistochemistry and western blotting. The proliferation ability of colorectal cancer cells was measured by colony formation assay and CCK-8 assay, the migration and invasion ability of colorectal cancer cells was measured by wound healing assay and transwell assay, and the protein expression levels of the Hippo signaling pathway and its target gene were examined by western blotting. Immunoprecipitation was used to assess the interaction of RNF128 with MST. In vivo, a xenograft tumor model was used to detect the effect of RNF128 on tumor growth. RESULTS: At the tissue level, the expression level of RNF128 was significantly higher in colorectal cancer tissues than in adjacent normal tissues. In LoVo cells and HCT116 cells, the proliferation, migration and invasion abilities were significantly reduced with RNF128 knockdown. At the protein level, knockdown of RNF128 resulted in significant activation of the Hippo signaling pathway. In vivo experiments, the volume and weight of xenograft tumors in nude mice were significantly decreased compared with those in the normal control group with RNF128 knockdown. CONCLUSION: RNF128 promotes the malignant behaviors of colorectal cancer cells by inhibiting the Hippo signaling pathway, which may provide a new target for colorectal cancer prevention and treatment.
format Online
Article
Text
id pubmed-9837830
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98378302023-01-14 Ring finger protein 128 promotes, rather than inhibits, colorectal cancer progression by regulating the Hippo signaling pathway Ning, Shili Chen, Yuzhuo Wang, Guangzhi Liu, Yongtai Yang, Yingchi Zhang, Zhongtao Front Oncol Oncology BACKGROUND: Colorectal cancer is a common malignancy of the gastrointestinal tract, and its incidence and mortality rates have increased in recent years. RNF128 is an E3 ubiquitin ligase that plays an important role as a suppressor gene or oncogene in various cancers, but its mechanism in colorectal cancer is not yet clear. The aim of this study was to investigate the role and mechanism of RNF128 in colorectal cancer. METHODS: The expression of RNF128 in colorectal cancer tissues was assessed by immunohistochemistry and western blotting. The proliferation ability of colorectal cancer cells was measured by colony formation assay and CCK-8 assay, the migration and invasion ability of colorectal cancer cells was measured by wound healing assay and transwell assay, and the protein expression levels of the Hippo signaling pathway and its target gene were examined by western blotting. Immunoprecipitation was used to assess the interaction of RNF128 with MST. In vivo, a xenograft tumor model was used to detect the effect of RNF128 on tumor growth. RESULTS: At the tissue level, the expression level of RNF128 was significantly higher in colorectal cancer tissues than in adjacent normal tissues. In LoVo cells and HCT116 cells, the proliferation, migration and invasion abilities were significantly reduced with RNF128 knockdown. At the protein level, knockdown of RNF128 resulted in significant activation of the Hippo signaling pathway. In vivo experiments, the volume and weight of xenograft tumors in nude mice were significantly decreased compared with those in the normal control group with RNF128 knockdown. CONCLUSION: RNF128 promotes the malignant behaviors of colorectal cancer cells by inhibiting the Hippo signaling pathway, which may provide a new target for colorectal cancer prevention and treatment. Frontiers Media S.A. 2022-12-29 /pmc/articles/PMC9837830/ /pubmed/36644633 http://dx.doi.org/10.3389/fonc.2022.1031160 Text en Copyright © 2022 Ning, Chen, Wang, Liu, Yang and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Ning, Shili
Chen, Yuzhuo
Wang, Guangzhi
Liu, Yongtai
Yang, Yingchi
Zhang, Zhongtao
Ring finger protein 128 promotes, rather than inhibits, colorectal cancer progression by regulating the Hippo signaling pathway
title Ring finger protein 128 promotes, rather than inhibits, colorectal cancer progression by regulating the Hippo signaling pathway
title_full Ring finger protein 128 promotes, rather than inhibits, colorectal cancer progression by regulating the Hippo signaling pathway
title_fullStr Ring finger protein 128 promotes, rather than inhibits, colorectal cancer progression by regulating the Hippo signaling pathway
title_full_unstemmed Ring finger protein 128 promotes, rather than inhibits, colorectal cancer progression by regulating the Hippo signaling pathway
title_short Ring finger protein 128 promotes, rather than inhibits, colorectal cancer progression by regulating the Hippo signaling pathway
title_sort ring finger protein 128 promotes, rather than inhibits, colorectal cancer progression by regulating the hippo signaling pathway
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837830/
https://www.ncbi.nlm.nih.gov/pubmed/36644633
http://dx.doi.org/10.3389/fonc.2022.1031160
work_keys_str_mv AT ningshili ringfingerprotein128promotesratherthaninhibitscolorectalcancerprogressionbyregulatingthehipposignalingpathway
AT chenyuzhuo ringfingerprotein128promotesratherthaninhibitscolorectalcancerprogressionbyregulatingthehipposignalingpathway
AT wangguangzhi ringfingerprotein128promotesratherthaninhibitscolorectalcancerprogressionbyregulatingthehipposignalingpathway
AT liuyongtai ringfingerprotein128promotesratherthaninhibitscolorectalcancerprogressionbyregulatingthehipposignalingpathway
AT yangyingchi ringfingerprotein128promotesratherthaninhibitscolorectalcancerprogressionbyregulatingthehipposignalingpathway
AT zhangzhongtao ringfingerprotein128promotesratherthaninhibitscolorectalcancerprogressionbyregulatingthehipposignalingpathway