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Atomic-Resolution Structure of the Protein Encoded by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined by Magic-Angle Spinning Solid-State NMR
[Image: see text] F-specific filamentous phages, elongated particles with circular single-stranded DNA encased in a symmetric protein capsid, undergo an intermediate step, where thousands of homodimers of a non-structural protein, gVp, bind to newly synthesized strands of DNA, preventing further DNA...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837838/ https://www.ncbi.nlm.nih.gov/pubmed/36542094 http://dx.doi.org/10.1021/jacs.2c09957 |
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author | Shamir, Yoav Goldbourt, Amir |
author_facet | Shamir, Yoav Goldbourt, Amir |
author_sort | Shamir, Yoav |
collection | PubMed |
description | [Image: see text] F-specific filamentous phages, elongated particles with circular single-stranded DNA encased in a symmetric protein capsid, undergo an intermediate step, where thousands of homodimers of a non-structural protein, gVp, bind to newly synthesized strands of DNA, preventing further DNA replication and preparing the circular genome in an elongated conformation for assembly of a new virion structure at the membrane. While the structure of the free homodimer is known, the ssDNA-bound conformation has yet to be determined. We report an atomic-resolution structure of the gVp monomer bound to ssDNA of fd phage in the nucleoprotein complex elucidated via magic-angle spinning solid-state NMR. The model presents significant conformational changes with respect to the free form. These modifications facilitate the binding mechanism and possibly promote cooperative binding in the assembly of the gVp–ssDNA complex. |
format | Online Article Text |
id | pubmed-9837838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-98378382023-01-14 Atomic-Resolution Structure of the Protein Encoded by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined by Magic-Angle Spinning Solid-State NMR Shamir, Yoav Goldbourt, Amir J Am Chem Soc [Image: see text] F-specific filamentous phages, elongated particles with circular single-stranded DNA encased in a symmetric protein capsid, undergo an intermediate step, where thousands of homodimers of a non-structural protein, gVp, bind to newly synthesized strands of DNA, preventing further DNA replication and preparing the circular genome in an elongated conformation for assembly of a new virion structure at the membrane. While the structure of the free homodimer is known, the ssDNA-bound conformation has yet to be determined. We report an atomic-resolution structure of the gVp monomer bound to ssDNA of fd phage in the nucleoprotein complex elucidated via magic-angle spinning solid-state NMR. The model presents significant conformational changes with respect to the free form. These modifications facilitate the binding mechanism and possibly promote cooperative binding in the assembly of the gVp–ssDNA complex. American Chemical Society 2022-12-21 /pmc/articles/PMC9837838/ /pubmed/36542094 http://dx.doi.org/10.1021/jacs.2c09957 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Shamir, Yoav Goldbourt, Amir Atomic-Resolution Structure of the Protein Encoded by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined by Magic-Angle Spinning Solid-State NMR |
title | Atomic-Resolution Structure
of the Protein Encoded
by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined
by Magic-Angle Spinning Solid-State NMR |
title_full | Atomic-Resolution Structure
of the Protein Encoded
by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined
by Magic-Angle Spinning Solid-State NMR |
title_fullStr | Atomic-Resolution Structure
of the Protein Encoded
by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined
by Magic-Angle Spinning Solid-State NMR |
title_full_unstemmed | Atomic-Resolution Structure
of the Protein Encoded
by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined
by Magic-Angle Spinning Solid-State NMR |
title_short | Atomic-Resolution Structure
of the Protein Encoded
by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined
by Magic-Angle Spinning Solid-State NMR |
title_sort | atomic-resolution structure
of the protein encoded
by gene v of fd bacteriophage in complex with viral ssdna determined
by magic-angle spinning solid-state nmr |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837838/ https://www.ncbi.nlm.nih.gov/pubmed/36542094 http://dx.doi.org/10.1021/jacs.2c09957 |
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