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No detectable changes in reproductive behaviour of Caenorhabditis elegans males after 97 generations under obligatory outcrossing

In Caenorhabditis elegans, a species reproducing mostly via self-fertilization, numerous signatures of selfing syndrome are observed, including differences in reproductive behaviour compared to related obligatory outcrossing species. In this study we investigated the effect of nearly 100 generations...

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Autores principales: Antoł, Weronika, Byszko, Jagoda, Dyba, Alicja, Palka, Joanna, Babik, Wiesław, Prokop, Zofia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9838211/
https://www.ncbi.nlm.nih.gov/pubmed/36643640
http://dx.doi.org/10.7717/peerj.14572
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author Antoł, Weronika
Byszko, Jagoda
Dyba, Alicja
Palka, Joanna
Babik, Wiesław
Prokop, Zofia
author_facet Antoł, Weronika
Byszko, Jagoda
Dyba, Alicja
Palka, Joanna
Babik, Wiesław
Prokop, Zofia
author_sort Antoł, Weronika
collection PubMed
description In Caenorhabditis elegans, a species reproducing mostly via self-fertilization, numerous signatures of selfing syndrome are observed, including differences in reproductive behaviour compared to related obligatory outcrossing species. In this study we investigated the effect of nearly 100 generations of obligatory outcrossing on several characteristics of male reproductive behaviour. A genetically uniform ancestral population carrying a mutation changing the reproductive system to obligatory outcrossing was split into four independent populations. We predicted that the transition from the natural reproductive system, where males were extremely rare, to obligatory outcrossing, where males comprise 50% of the population and are necessary for reproduction, will increase the selection pressure on higher effectiveness of mating behaviour. Several characteristics of male mating behaviour during a 15 min interaction as well as copulation success were compared between the ancestral and evolved populations. No significant differences in male mating behaviour or fertilization success were detected between generations 1 and 97 of obligatory outcrossing populations. We found, however, that longer contact with females increased chances of successful copulation, although this effect did not differ between populations. We conclude that either selection acting on male mating behaviour has not been strong enough, or mutational input of new adaptive variants has not been sufficient to cause noticeable behavioural differences after 97 generations of evolution starting from genetically uniform population.
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spelling pubmed-98382112023-01-14 No detectable changes in reproductive behaviour of Caenorhabditis elegans males after 97 generations under obligatory outcrossing Antoł, Weronika Byszko, Jagoda Dyba, Alicja Palka, Joanna Babik, Wiesław Prokop, Zofia PeerJ Animal Behavior In Caenorhabditis elegans, a species reproducing mostly via self-fertilization, numerous signatures of selfing syndrome are observed, including differences in reproductive behaviour compared to related obligatory outcrossing species. In this study we investigated the effect of nearly 100 generations of obligatory outcrossing on several characteristics of male reproductive behaviour. A genetically uniform ancestral population carrying a mutation changing the reproductive system to obligatory outcrossing was split into four independent populations. We predicted that the transition from the natural reproductive system, where males were extremely rare, to obligatory outcrossing, where males comprise 50% of the population and are necessary for reproduction, will increase the selection pressure on higher effectiveness of mating behaviour. Several characteristics of male mating behaviour during a 15 min interaction as well as copulation success were compared between the ancestral and evolved populations. No significant differences in male mating behaviour or fertilization success were detected between generations 1 and 97 of obligatory outcrossing populations. We found, however, that longer contact with females increased chances of successful copulation, although this effect did not differ between populations. We conclude that either selection acting on male mating behaviour has not been strong enough, or mutational input of new adaptive variants has not been sufficient to cause noticeable behavioural differences after 97 generations of evolution starting from genetically uniform population. PeerJ Inc. 2023-01-10 /pmc/articles/PMC9838211/ /pubmed/36643640 http://dx.doi.org/10.7717/peerj.14572 Text en © 2023 Antoł et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Animal Behavior
Antoł, Weronika
Byszko, Jagoda
Dyba, Alicja
Palka, Joanna
Babik, Wiesław
Prokop, Zofia
No detectable changes in reproductive behaviour of Caenorhabditis elegans males after 97 generations under obligatory outcrossing
title No detectable changes in reproductive behaviour of Caenorhabditis elegans males after 97 generations under obligatory outcrossing
title_full No detectable changes in reproductive behaviour of Caenorhabditis elegans males after 97 generations under obligatory outcrossing
title_fullStr No detectable changes in reproductive behaviour of Caenorhabditis elegans males after 97 generations under obligatory outcrossing
title_full_unstemmed No detectable changes in reproductive behaviour of Caenorhabditis elegans males after 97 generations under obligatory outcrossing
title_short No detectable changes in reproductive behaviour of Caenorhabditis elegans males after 97 generations under obligatory outcrossing
title_sort no detectable changes in reproductive behaviour of caenorhabditis elegans males after 97 generations under obligatory outcrossing
topic Animal Behavior
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9838211/
https://www.ncbi.nlm.nih.gov/pubmed/36643640
http://dx.doi.org/10.7717/peerj.14572
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