Multi-amplicon microbiome data analysis pipelines for mixed orientation sequences using QIIME2: Assessing reference database, variable region and pre-processing bias in classification of mock bacterial community samples

Microbiome research relies on next-generation sequencing and on downstream data analysis workflows. Several manufacturers have introduced multi-amplicon kits for microbiome characterization, improving speciation, but present unique challenges for analysis. The goal of this methodology study was to d...

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Detalles Bibliográficos
Autores principales: Maki, Katherine A., Wolff, Brian, Varuzza, Leonardo, Green, Stefan J., Barb, Jennifer J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9838852/
https://www.ncbi.nlm.nih.gov/pubmed/36638095
http://dx.doi.org/10.1371/journal.pone.0280293

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9838852/
https://www.ncbi.nlm.nih.gov/pubmed/36638095
http://dx.doi.org/10.1371/journal.pone.0280293

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