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Longitudinal proteomic profiling of the inflammatory response in dengue patients
BACKGROUND: The immunopathogenesis of dengue virus (DENV) infection remains incompletely understood. To increase our understanding of inflammatory response in non-severe dengue, we assessed longitudinal changes in the inflammatory proteome in patients with an acute DENV infection. METHODS: Using a m...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9838874/ https://www.ncbi.nlm.nih.gov/pubmed/36595532 http://dx.doi.org/10.1371/journal.pntd.0011041 |
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author | Garishah, Fadel Muhammad Boahen, Collins K. Vadaq, Nadira Pramudo, Setyo G. Tunjungputri, Rahajeng N. Riswari, Silvita Fitri van Rij, Ronald P. Alisjahbana, Bachti Gasem, Muhammad Hussein van der Ven, André J. A. M. de Mast, Quirijn |
author_facet | Garishah, Fadel Muhammad Boahen, Collins K. Vadaq, Nadira Pramudo, Setyo G. Tunjungputri, Rahajeng N. Riswari, Silvita Fitri van Rij, Ronald P. Alisjahbana, Bachti Gasem, Muhammad Hussein van der Ven, André J. A. M. de Mast, Quirijn |
author_sort | Garishah, Fadel Muhammad |
collection | PubMed |
description | BACKGROUND: The immunopathogenesis of dengue virus (DENV) infection remains incompletely understood. To increase our understanding of inflammatory response in non-severe dengue, we assessed longitudinal changes in the inflammatory proteome in patients with an acute DENV infection. METHODS: Using a multiplex proximity extension assay (PEA), we measured relative levels of 368 inflammatory markers in plasma samples from hospitalized patients with non-severe DENV infection in the acute (n = 43) and convalescence (n = 35) phase of the infection and samples of healthy controls (n = 10). RESULTS: We identified 203 upregulated and 39 downregulated proteins in acute versus convalescent plasma samples. The upregulated proteins had a strong representation of interferon (IFN) and IFN-inducible effector proteins, cytokines (e.g. IL-10, IL-33) and cytokine receptors, chemokines, pro-apoptotic proteins (e.g. granzymes) and endothelial markers. A number of differentially expressed proteins (DEPs) have not been reported in previous studies. Functional network analysis highlighted a central role for IFNγ, IL-10, IL-33 and chemokines. We identified different novel associations between inflammatory proteins and circulating concentrations of the endothelial glycocalyx disruption surrogate marker syndecan-1. Conclusion: This unbiased proteome analysis provides a comprehensive insight in the inflammatory response in DENV infection and its association with glycocalyx disruption. |
format | Online Article Text |
id | pubmed-9838874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-98388742023-01-14 Longitudinal proteomic profiling of the inflammatory response in dengue patients Garishah, Fadel Muhammad Boahen, Collins K. Vadaq, Nadira Pramudo, Setyo G. Tunjungputri, Rahajeng N. Riswari, Silvita Fitri van Rij, Ronald P. Alisjahbana, Bachti Gasem, Muhammad Hussein van der Ven, André J. A. M. de Mast, Quirijn PLoS Negl Trop Dis Research Article BACKGROUND: The immunopathogenesis of dengue virus (DENV) infection remains incompletely understood. To increase our understanding of inflammatory response in non-severe dengue, we assessed longitudinal changes in the inflammatory proteome in patients with an acute DENV infection. METHODS: Using a multiplex proximity extension assay (PEA), we measured relative levels of 368 inflammatory markers in plasma samples from hospitalized patients with non-severe DENV infection in the acute (n = 43) and convalescence (n = 35) phase of the infection and samples of healthy controls (n = 10). RESULTS: We identified 203 upregulated and 39 downregulated proteins in acute versus convalescent plasma samples. The upregulated proteins had a strong representation of interferon (IFN) and IFN-inducible effector proteins, cytokines (e.g. IL-10, IL-33) and cytokine receptors, chemokines, pro-apoptotic proteins (e.g. granzymes) and endothelial markers. A number of differentially expressed proteins (DEPs) have not been reported in previous studies. Functional network analysis highlighted a central role for IFNγ, IL-10, IL-33 and chemokines. We identified different novel associations between inflammatory proteins and circulating concentrations of the endothelial glycocalyx disruption surrogate marker syndecan-1. Conclusion: This unbiased proteome analysis provides a comprehensive insight in the inflammatory response in DENV infection and its association with glycocalyx disruption. Public Library of Science 2023-01-03 /pmc/articles/PMC9838874/ /pubmed/36595532 http://dx.doi.org/10.1371/journal.pntd.0011041 Text en © 2023 Garishah et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Garishah, Fadel Muhammad Boahen, Collins K. Vadaq, Nadira Pramudo, Setyo G. Tunjungputri, Rahajeng N. Riswari, Silvita Fitri van Rij, Ronald P. Alisjahbana, Bachti Gasem, Muhammad Hussein van der Ven, André J. A. M. de Mast, Quirijn Longitudinal proteomic profiling of the inflammatory response in dengue patients |
title | Longitudinal proteomic profiling of the inflammatory response in dengue patients |
title_full | Longitudinal proteomic profiling of the inflammatory response in dengue patients |
title_fullStr | Longitudinal proteomic profiling of the inflammatory response in dengue patients |
title_full_unstemmed | Longitudinal proteomic profiling of the inflammatory response in dengue patients |
title_short | Longitudinal proteomic profiling of the inflammatory response in dengue patients |
title_sort | longitudinal proteomic profiling of the inflammatory response in dengue patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9838874/ https://www.ncbi.nlm.nih.gov/pubmed/36595532 http://dx.doi.org/10.1371/journal.pntd.0011041 |
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