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Enhanced surface nanoanalytics of transient biomolecular processes

Fundamental knowledge of the physical and chemical properties of biomolecules is key to understanding molecular processes in health and disease. Bulk and single-molecule analytical methods provide rich information about biomolecules but often require high concentrations and sample preparation away f...

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Autores principales: Miller, Alyssa, Chia, Sean, Toprakcioglu, Zenon, Hakala, Tuuli, Schmid, Roman, Feng, Yaduo, Kartanas, Tadas, Kamada, Ayaka, Vendruscolo, Michele, Ruggeri, Francesco Simone, Knowles, Tuomas P. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9839325/
https://www.ncbi.nlm.nih.gov/pubmed/36638180
http://dx.doi.org/10.1126/sciadv.abq3151
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author Miller, Alyssa
Chia, Sean
Toprakcioglu, Zenon
Hakala, Tuuli
Schmid, Roman
Feng, Yaduo
Kartanas, Tadas
Kamada, Ayaka
Vendruscolo, Michele
Ruggeri, Francesco Simone
Knowles, Tuomas P. J.
author_facet Miller, Alyssa
Chia, Sean
Toprakcioglu, Zenon
Hakala, Tuuli
Schmid, Roman
Feng, Yaduo
Kartanas, Tadas
Kamada, Ayaka
Vendruscolo, Michele
Ruggeri, Francesco Simone
Knowles, Tuomas P. J.
author_sort Miller, Alyssa
collection PubMed
description Fundamental knowledge of the physical and chemical properties of biomolecules is key to understanding molecular processes in health and disease. Bulk and single-molecule analytical methods provide rich information about biomolecules but often require high concentrations and sample preparation away from physiologically relevant conditions. Here, we present the development and application of a lab-on-a-chip spray approach that combines rapid sample preparation, mixing, and deposition to integrate with a range of nanoanalytical methods in chemistry and biology, providing enhanced spectroscopic sensitivity and single-molecule spatial resolution. We demonstrate that this method enables multidimensional study of heterogeneous biomolecular systems over multiple length scales by nanoscopy and vibrational spectroscopy. We then illustrate the capabilities of this platform by capturing and analyzing the structural conformations of transient oligomeric species formed at the early stages of the self-assembly of α-synuclein, which are associated with the onset of Parkinson’s disease.
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spelling pubmed-98393252023-01-30 Enhanced surface nanoanalytics of transient biomolecular processes Miller, Alyssa Chia, Sean Toprakcioglu, Zenon Hakala, Tuuli Schmid, Roman Feng, Yaduo Kartanas, Tadas Kamada, Ayaka Vendruscolo, Michele Ruggeri, Francesco Simone Knowles, Tuomas P. J. Sci Adv Physical and Materials Sciences Fundamental knowledge of the physical and chemical properties of biomolecules is key to understanding molecular processes in health and disease. Bulk and single-molecule analytical methods provide rich information about biomolecules but often require high concentrations and sample preparation away from physiologically relevant conditions. Here, we present the development and application of a lab-on-a-chip spray approach that combines rapid sample preparation, mixing, and deposition to integrate with a range of nanoanalytical methods in chemistry and biology, providing enhanced spectroscopic sensitivity and single-molecule spatial resolution. We demonstrate that this method enables multidimensional study of heterogeneous biomolecular systems over multiple length scales by nanoscopy and vibrational spectroscopy. We then illustrate the capabilities of this platform by capturing and analyzing the structural conformations of transient oligomeric species formed at the early stages of the self-assembly of α-synuclein, which are associated with the onset of Parkinson’s disease. American Association for the Advancement of Science 2023-01-13 /pmc/articles/PMC9839325/ /pubmed/36638180 http://dx.doi.org/10.1126/sciadv.abq3151 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Physical and Materials Sciences
Miller, Alyssa
Chia, Sean
Toprakcioglu, Zenon
Hakala, Tuuli
Schmid, Roman
Feng, Yaduo
Kartanas, Tadas
Kamada, Ayaka
Vendruscolo, Michele
Ruggeri, Francesco Simone
Knowles, Tuomas P. J.
Enhanced surface nanoanalytics of transient biomolecular processes
title Enhanced surface nanoanalytics of transient biomolecular processes
title_full Enhanced surface nanoanalytics of transient biomolecular processes
title_fullStr Enhanced surface nanoanalytics of transient biomolecular processes
title_full_unstemmed Enhanced surface nanoanalytics of transient biomolecular processes
title_short Enhanced surface nanoanalytics of transient biomolecular processes
title_sort enhanced surface nanoanalytics of transient biomolecular processes
topic Physical and Materials Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9839325/
https://www.ncbi.nlm.nih.gov/pubmed/36638180
http://dx.doi.org/10.1126/sciadv.abq3151
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