Cargando…

Obese dogs exhibit different fecal microbiome and specific microbial networks compared with normal weight dogs

Canine obesity is a major health concern that predisposes dogs to various disorders. The fecal microbiome has been attracting attention because of their impact on energy efficiency and metabolic disorders of host. However, little is known about specific microbial interactions, and how these may be a...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Hanbeen, Seo, Jakyeom, Park, Tansol, Seo, Kangmin, Cho, Hyun-Woo, Chun, Ju Lan, Kim, Ki Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9839755/
https://www.ncbi.nlm.nih.gov/pubmed/36639715
http://dx.doi.org/10.1038/s41598-023-27846-3
_version_ 1784869513357950976
author Kim, Hanbeen
Seo, Jakyeom
Park, Tansol
Seo, Kangmin
Cho, Hyun-Woo
Chun, Ju Lan
Kim, Ki Hyun
author_facet Kim, Hanbeen
Seo, Jakyeom
Park, Tansol
Seo, Kangmin
Cho, Hyun-Woo
Chun, Ju Lan
Kim, Ki Hyun
author_sort Kim, Hanbeen
collection PubMed
description Canine obesity is a major health concern that predisposes dogs to various disorders. The fecal microbiome has been attracting attention because of their impact on energy efficiency and metabolic disorders of host. However, little is known about specific microbial interactions, and how these may be affected by obesity in dogs. The objective of this study was to investigate the differences in fecal microbiome and specific microbial networks between obese and normal dogs. A total of 20 beagle dogs (males = 12, body weight [BW]: 10.5 ± 1.08 kg; females = 8, BW: 11.3 ± 1.71 kg; all 2-year-old) were fed to meet the maintenance energy requirements for 18 weeks. Then, 12 beagle dogs were selected based on body condition score (BCS) and divided into two groups: high BCS group (HBCS; BCS range: 7–9, males = 4, females = 2) and normal BCS group (NBCS; BCS range: 4–6, males = 4, females = 2). In the final week of the experiment, fecal samples were collected directly from the rectum, before breakfast, for analyzing the fecal microbiome using 16S rRNA gene amplicon sequencing. The HBCS group had a significantly higher final BW than the NBCS group (P < 0.01). The relative abundances of Faecalibacterium, Phascolarctobacterium, Megamonas, Bacteroides, Mucispirillum, and an unclassified genus within Ruminococcaceae were significantly higher in the HBCS group than those in the NBCS group (P < 0.05). Furthermore, some Kyoto Encyclopedia of Genes and Genomes (KEGG) modules related to amino acid biosynthesis and B vitamins biosynthesis were enriched in the HBCS group (P < 0.10), whereas those related to carbohydrate metabolism were enriched in the NBCS group (P < 0.10). Microbial network analysis revealed distinct co-occurrence and mutually exclusive interactions between the HBCS and NBCS groups. In conclusion, several genera related to short-chain fatty acid production were enriched in the HBCS group. The enriched KEGG modules in the HBCS group enhanced energy efficiency through cross-feeding between auxotrophs and prototrophs. However, further studies are needed to investigate how specific networks can be interpreted in the context of fermentation characteristics in the lower gut and obesity in dogs.
format Online
Article
Text
id pubmed-9839755
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-98397552023-01-15 Obese dogs exhibit different fecal microbiome and specific microbial networks compared with normal weight dogs Kim, Hanbeen Seo, Jakyeom Park, Tansol Seo, Kangmin Cho, Hyun-Woo Chun, Ju Lan Kim, Ki Hyun Sci Rep Article Canine obesity is a major health concern that predisposes dogs to various disorders. The fecal microbiome has been attracting attention because of their impact on energy efficiency and metabolic disorders of host. However, little is known about specific microbial interactions, and how these may be affected by obesity in dogs. The objective of this study was to investigate the differences in fecal microbiome and specific microbial networks between obese and normal dogs. A total of 20 beagle dogs (males = 12, body weight [BW]: 10.5 ± 1.08 kg; females = 8, BW: 11.3 ± 1.71 kg; all 2-year-old) were fed to meet the maintenance energy requirements for 18 weeks. Then, 12 beagle dogs were selected based on body condition score (BCS) and divided into two groups: high BCS group (HBCS; BCS range: 7–9, males = 4, females = 2) and normal BCS group (NBCS; BCS range: 4–6, males = 4, females = 2). In the final week of the experiment, fecal samples were collected directly from the rectum, before breakfast, for analyzing the fecal microbiome using 16S rRNA gene amplicon sequencing. The HBCS group had a significantly higher final BW than the NBCS group (P < 0.01). The relative abundances of Faecalibacterium, Phascolarctobacterium, Megamonas, Bacteroides, Mucispirillum, and an unclassified genus within Ruminococcaceae were significantly higher in the HBCS group than those in the NBCS group (P < 0.05). Furthermore, some Kyoto Encyclopedia of Genes and Genomes (KEGG) modules related to amino acid biosynthesis and B vitamins biosynthesis were enriched in the HBCS group (P < 0.10), whereas those related to carbohydrate metabolism were enriched in the NBCS group (P < 0.10). Microbial network analysis revealed distinct co-occurrence and mutually exclusive interactions between the HBCS and NBCS groups. In conclusion, several genera related to short-chain fatty acid production were enriched in the HBCS group. The enriched KEGG modules in the HBCS group enhanced energy efficiency through cross-feeding between auxotrophs and prototrophs. However, further studies are needed to investigate how specific networks can be interpreted in the context of fermentation characteristics in the lower gut and obesity in dogs. Nature Publishing Group UK 2023-01-13 /pmc/articles/PMC9839755/ /pubmed/36639715 http://dx.doi.org/10.1038/s41598-023-27846-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kim, Hanbeen
Seo, Jakyeom
Park, Tansol
Seo, Kangmin
Cho, Hyun-Woo
Chun, Ju Lan
Kim, Ki Hyun
Obese dogs exhibit different fecal microbiome and specific microbial networks compared with normal weight dogs
title Obese dogs exhibit different fecal microbiome and specific microbial networks compared with normal weight dogs
title_full Obese dogs exhibit different fecal microbiome and specific microbial networks compared with normal weight dogs
title_fullStr Obese dogs exhibit different fecal microbiome and specific microbial networks compared with normal weight dogs
title_full_unstemmed Obese dogs exhibit different fecal microbiome and specific microbial networks compared with normal weight dogs
title_short Obese dogs exhibit different fecal microbiome and specific microbial networks compared with normal weight dogs
title_sort obese dogs exhibit different fecal microbiome and specific microbial networks compared with normal weight dogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9839755/
https://www.ncbi.nlm.nih.gov/pubmed/36639715
http://dx.doi.org/10.1038/s41598-023-27846-3
work_keys_str_mv AT kimhanbeen obesedogsexhibitdifferentfecalmicrobiomeandspecificmicrobialnetworkscomparedwithnormalweightdogs
AT seojakyeom obesedogsexhibitdifferentfecalmicrobiomeandspecificmicrobialnetworkscomparedwithnormalweightdogs
AT parktansol obesedogsexhibitdifferentfecalmicrobiomeandspecificmicrobialnetworkscomparedwithnormalweightdogs
AT seokangmin obesedogsexhibitdifferentfecalmicrobiomeandspecificmicrobialnetworkscomparedwithnormalweightdogs
AT chohyunwoo obesedogsexhibitdifferentfecalmicrobiomeandspecificmicrobialnetworkscomparedwithnormalweightdogs
AT chunjulan obesedogsexhibitdifferentfecalmicrobiomeandspecificmicrobialnetworkscomparedwithnormalweightdogs
AT kimkihyun obesedogsexhibitdifferentfecalmicrobiomeandspecificmicrobialnetworkscomparedwithnormalweightdogs