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Examination of human osteoarchaeological remains as a feasible source of polar and apolar metabolites to study past conditions

Metabolomics is a modern tool that aids in our understanding of the molecular changes in organisms. Archaeological science is a branch of archaeology that explores different archaeological materials using modern analytical tools. Human osteoarchaeological material are a frequent finding in archaeolo...

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Detalles Bibliográficos
Autores principales: Badillo-Sanchez, Diego, Ruber, Maria Serrano, Davies-Barrett, Anna M., Sandhu, Jatinderpal K., Jones, Donald J. L., Hansen, Martin, Inskip, Sarah A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9839756/
https://www.ncbi.nlm.nih.gov/pubmed/36639564
http://dx.doi.org/10.1038/s41598-023-27401-0
Descripción
Sumario:Metabolomics is a modern tool that aids in our understanding of the molecular changes in organisms. Archaeological science is a branch of archaeology that explores different archaeological materials using modern analytical tools. Human osteoarchaeological material are a frequent finding in archaeological contexts and have the potential to offer information about previous human populations, which can be illuminating about our current condition. Using a set of samples comprising different skeletal elements and bone structures, here we explore for the first time the possibility of extracting metabolites from osteoarchaeological material. Here, a protocol for extraction and measurement of extracted polar and less-polar/apolar metabolites by ultra-high performance liquid chromatography hyphenated to high resolution mass spectrometry is presented to measure the molecules separated after a reversed phase and hydrophilic interaction liquid chromatography column. Molecular information was obtained, showing that osteoarchaeological material is a viable source of molecular information for metabolomic studies.