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Rapid and label-free histological imaging of unprocessed surgical tissues via dark-field reflectance ultraviolet microscopy

Routine examination for intraoperative histopathologic assessment is lengthy and laborious. Here, we present the dark-field reflectance ultraviolet microscopy (DRUM) that enables label-free imaging of unprocessed and thick tissues with subcellular resolution and a high signal-to-background ratio. To...

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Detalles Bibliográficos
Autores principales: Ye, Shiwei, Zou, Junjie, Huang, Chenming, Xiang, Feng, Wen, Zonghua, Wang, Nannan, Yu, Jia, He, Yuezhi, Liu, Peng, Mei, Xin, Li, Hui, Niu, Lili, Gong, Peng, Zheng, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9839964/
https://www.ncbi.nlm.nih.gov/pubmed/36647380
http://dx.doi.org/10.1016/j.isci.2022.105849
Descripción
Sumario:Routine examination for intraoperative histopathologic assessment is lengthy and laborious. Here, we present the dark-field reflectance ultraviolet microscopy (DRUM) that enables label-free imaging of unprocessed and thick tissues with subcellular resolution and a high signal-to-background ratio. To the best of our knowledge, DRUM provides image results for pathological assessment with the shortest turnaround time (2-3 min in total from sample preparation to tissue imaging). We also proposed a virtual staining process to convert DRUM images into pseudo-colorized images and enhance the image familiarity of pathologists. By imaging various tissues, we found DRUM can resolve cell nuclei and some extranuclear features, which are comparable to standard H&E images. Furthermore, the essential diagnostic features of intraoperatively excised tumor tissues also can be revealed by DRUM, demonstrating its potential as an additional aid for intraoperative histopathology.