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Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels

The tissue engineering triad comprises the combination of cells, scaffolds and biological factors. Therefore, we prepared cell- and drug-loaded hydrogels using in situ silk fibroin (SF) hydrogels induced by dimyristoyl glycerophosphoglycerol (DMPG). DMPG is reported to induce rapid hydrogel formatio...

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Autores principales: Laomeephol, Chavee, Ferreira, Helena, Kanokpanont, Sorada, Luckanagul, Jittima Amie, Neves, Nuno M., Damrongsakkul, Siriporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese Medical Multimedia Press Co., Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9840088/
https://www.ncbi.nlm.nih.gov/pubmed/36654777
http://dx.doi.org/10.12336/biomatertransl.2022.03.005
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author Laomeephol, Chavee
Ferreira, Helena
Kanokpanont, Sorada
Luckanagul, Jittima Amie
Neves, Nuno M.
Damrongsakkul, Siriporn
author_facet Laomeephol, Chavee
Ferreira, Helena
Kanokpanont, Sorada
Luckanagul, Jittima Amie
Neves, Nuno M.
Damrongsakkul, Siriporn
author_sort Laomeephol, Chavee
collection PubMed
description The tissue engineering triad comprises the combination of cells, scaffolds and biological factors. Therefore, we prepared cell- and drug-loaded hydrogels using in situ silk fibroin (SF) hydrogels induced by dimyristoyl glycerophosphoglycerol (DMPG). DMPG is reported to induce rapid hydrogel formation by SF, facilitating cell encapsulation in the hydrogel matrix while maintaining high cell viability and proliferative capacity. In addition, DMPG can be used for liposome formulations in entrapping drug molecules. Dexamethasone (Dex) was loaded into the DMPG-induced SF hydrogels together with human osteoblast-like SaOS-2 cells, then the osteogenic differentiation of the entrapped cells was evaluated in vitro and compared to cells cultured under standard conditions. Calcium production by cells cultured in DMPG/Dex-SF hydrogels with Dex-depleted osteogenic medium was equivalent to that of cells cultured in conventional osteogenic medium containing Dex. The extended-release of the entrapped Dex by the hydrogels was able to provide a sufficient drug amount for osteogenic induction. The controlled release of Dex was also advantageous for cell viability even though its dose in the hydrogels was far higher than that in osteogenic medium. The results confirmed the possibility of using DMPG-induced SF hydrogels to enable dual cell and drug encapsulation to fulfil the practical applications of tissue-engineered constructs.
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spelling pubmed-98400882023-01-17 Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels Laomeephol, Chavee Ferreira, Helena Kanokpanont, Sorada Luckanagul, Jittima Amie Neves, Nuno M. Damrongsakkul, Siriporn Biomater Transl Research Article The tissue engineering triad comprises the combination of cells, scaffolds and biological factors. Therefore, we prepared cell- and drug-loaded hydrogels using in situ silk fibroin (SF) hydrogels induced by dimyristoyl glycerophosphoglycerol (DMPG). DMPG is reported to induce rapid hydrogel formation by SF, facilitating cell encapsulation in the hydrogel matrix while maintaining high cell viability and proliferative capacity. In addition, DMPG can be used for liposome formulations in entrapping drug molecules. Dexamethasone (Dex) was loaded into the DMPG-induced SF hydrogels together with human osteoblast-like SaOS-2 cells, then the osteogenic differentiation of the entrapped cells was evaluated in vitro and compared to cells cultured under standard conditions. Calcium production by cells cultured in DMPG/Dex-SF hydrogels with Dex-depleted osteogenic medium was equivalent to that of cells cultured in conventional osteogenic medium containing Dex. The extended-release of the entrapped Dex by the hydrogels was able to provide a sufficient drug amount for osteogenic induction. The controlled release of Dex was also advantageous for cell viability even though its dose in the hydrogels was far higher than that in osteogenic medium. The results confirmed the possibility of using DMPG-induced SF hydrogels to enable dual cell and drug encapsulation to fulfil the practical applications of tissue-engineered constructs. Chinese Medical Multimedia Press Co., Ltd 2022-09-28 /pmc/articles/PMC9840088/ /pubmed/36654777 http://dx.doi.org/10.12336/biomatertransl.2022.03.005 Text en https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work noncommercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Research Article
Laomeephol, Chavee
Ferreira, Helena
Kanokpanont, Sorada
Luckanagul, Jittima Amie
Neves, Nuno M.
Damrongsakkul, Siriporn
Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels
title Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels
title_full Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels
title_fullStr Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels
title_full_unstemmed Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels
title_short Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels
title_sort osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9840088/
https://www.ncbi.nlm.nih.gov/pubmed/36654777
http://dx.doi.org/10.12336/biomatertransl.2022.03.005
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