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Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes

The human pathogen Listeria monocytogenes can cope with severe environmental challenges, for which the high molecular weight stressosome complex acts as the sensing hub in a complicated signal transduction pathway. Here, we show the dynamics and functional roles of the stressosome protein RsbR1 and...

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Detalles Bibliográficos
Autores principales: Tran, Buu Minh, Linnik, Dmitrii Sergeevich, Punter, Christiaan Michiel, Śmigiel, Wojciech Mikołaj, Mantovanelli, Luca, Iyer, Aditya, O’Byrne, Conor, Abee, Tjakko, Johansson, Jörgen, Poolman, Bert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9840623/
https://www.ncbi.nlm.nih.gov/pubmed/36641529
http://dx.doi.org/10.1038/s42003-023-04423-y
Descripción
Sumario:The human pathogen Listeria monocytogenes can cope with severe environmental challenges, for which the high molecular weight stressosome complex acts as the sensing hub in a complicated signal transduction pathway. Here, we show the dynamics and functional roles of the stressosome protein RsbR1 and its paralogue, the blue-light receptor RsbL, using photo-activated localization microscopy combined with single-particle tracking and single-molecule displacement mapping and supported by physiological studies. In live cells, RsbR1 is present in multiple states: in protomers with RsbS, large clusters of stressosome complexes, and in connection with the plasma membrane via Prli42. RsbL diffuses freely in the cytoplasm but forms clusters upon exposure to light. The clustering of RsbL is independent of the presence of Prli42. Our work provides a comprehensive view of the spatial organization and intracellular dynamics of the stressosome proteins in L. monocytogenes, which paves the way towards uncovering the stress-sensing mechanism of this signal transduction pathway.