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Effect of GATAD1 regulating the SRRM2 gene on recurrence of thyroid tumor and its molecular mechanism

BACKGROUND: Thyroid blood vessels and nerves are rich, and their anatomical and physiological structures are complex. Surgery often fails to eradicate the tumor, which has a serious negative impact on the surgical outcomes and patient prognosis. Therefore, it is important to accurately predict the r...

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Autores principales: Wang, Xingquan, Su, Dewang, Wei, Yaqing, Liu, Shilin, Gao, Shengyu, Tian, Hao, Wei, Weiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841002/
https://www.ncbi.nlm.nih.gov/pubmed/36654960
http://dx.doi.org/10.21037/gs-22-666
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author Wang, Xingquan
Su, Dewang
Wei, Yaqing
Liu, Shilin
Gao, Shengyu
Tian, Hao
Wei, Weiwei
author_facet Wang, Xingquan
Su, Dewang
Wei, Yaqing
Liu, Shilin
Gao, Shengyu
Tian, Hao
Wei, Weiwei
author_sort Wang, Xingquan
collection PubMed
description BACKGROUND: Thyroid blood vessels and nerves are rich, and their anatomical and physiological structures are complex. Surgery often fails to eradicate the tumor, which has a serious negative impact on the surgical outcomes and patient prognosis. Therefore, it is important to accurately predict the recurrence rate of thyroid cancer. METHODS: Based on bioinformatics analysis, the highly expressed transcription factors and differential genes in thyroid carcinoma (THCA) were obtained. Kaplan-Meier survival analysis was used to analyze the clinical effects of GATAD1 as well as SRRM2 on the recurrence of THCA patients. The effect of GATAD1 on SRRM2 expression was explored using cell experiments. Other experiments were conducted to reveal the interaction between SRRM2 and GATAD1 and their functions in THCA progression, such as cell proliferation and cell cycle. RESULTS: GATAD1 was overexpressed in recurrent THCA tissue compared with that in adjacent normal tissue. GATAD1 and SRRM2 were identified as the key risk factors for THCA recurrence as well as survival. Knockdown of GATAD1 and SRRM2 can inhibit THCA cell proliferation and arrest THCA cells in the G1 phase. Inhibiting GATAD1 decreased SRRM2 expression in THCA cells, whereas overexpressing GATAD1 had the opposite result. SRRM2 knockdown eliminated GATAD1-induced proliferation of THCA cells in vitro, indicating that GATAD1-induced THCA cell proliferation was dependent on increased SRRM2 expression. CONCLUSIONS: We identified GATAD1 as an underlying diagnostic biomarker in THCA recurrence patients. The GATAD1-SRRM2 axis mediates human THCA recurrence progression and is an underlying target for THCA treatment.
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spelling pubmed-98410022023-01-17 Effect of GATAD1 regulating the SRRM2 gene on recurrence of thyroid tumor and its molecular mechanism Wang, Xingquan Su, Dewang Wei, Yaqing Liu, Shilin Gao, Shengyu Tian, Hao Wei, Weiwei Gland Surg Original Article BACKGROUND: Thyroid blood vessels and nerves are rich, and their anatomical and physiological structures are complex. Surgery often fails to eradicate the tumor, which has a serious negative impact on the surgical outcomes and patient prognosis. Therefore, it is important to accurately predict the recurrence rate of thyroid cancer. METHODS: Based on bioinformatics analysis, the highly expressed transcription factors and differential genes in thyroid carcinoma (THCA) were obtained. Kaplan-Meier survival analysis was used to analyze the clinical effects of GATAD1 as well as SRRM2 on the recurrence of THCA patients. The effect of GATAD1 on SRRM2 expression was explored using cell experiments. Other experiments were conducted to reveal the interaction between SRRM2 and GATAD1 and their functions in THCA progression, such as cell proliferation and cell cycle. RESULTS: GATAD1 was overexpressed in recurrent THCA tissue compared with that in adjacent normal tissue. GATAD1 and SRRM2 were identified as the key risk factors for THCA recurrence as well as survival. Knockdown of GATAD1 and SRRM2 can inhibit THCA cell proliferation and arrest THCA cells in the G1 phase. Inhibiting GATAD1 decreased SRRM2 expression in THCA cells, whereas overexpressing GATAD1 had the opposite result. SRRM2 knockdown eliminated GATAD1-induced proliferation of THCA cells in vitro, indicating that GATAD1-induced THCA cell proliferation was dependent on increased SRRM2 expression. CONCLUSIONS: We identified GATAD1 as an underlying diagnostic biomarker in THCA recurrence patients. The GATAD1-SRRM2 axis mediates human THCA recurrence progression and is an underlying target for THCA treatment. AME Publishing Company 2022-12 /pmc/articles/PMC9841002/ /pubmed/36654960 http://dx.doi.org/10.21037/gs-22-666 Text en 2022 Gland Surgery. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Wang, Xingquan
Su, Dewang
Wei, Yaqing
Liu, Shilin
Gao, Shengyu
Tian, Hao
Wei, Weiwei
Effect of GATAD1 regulating the SRRM2 gene on recurrence of thyroid tumor and its molecular mechanism
title Effect of GATAD1 regulating the SRRM2 gene on recurrence of thyroid tumor and its molecular mechanism
title_full Effect of GATAD1 regulating the SRRM2 gene on recurrence of thyroid tumor and its molecular mechanism
title_fullStr Effect of GATAD1 regulating the SRRM2 gene on recurrence of thyroid tumor and its molecular mechanism
title_full_unstemmed Effect of GATAD1 regulating the SRRM2 gene on recurrence of thyroid tumor and its molecular mechanism
title_short Effect of GATAD1 regulating the SRRM2 gene on recurrence of thyroid tumor and its molecular mechanism
title_sort effect of gatad1 regulating the srrm2 gene on recurrence of thyroid tumor and its molecular mechanism
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841002/
https://www.ncbi.nlm.nih.gov/pubmed/36654960
http://dx.doi.org/10.21037/gs-22-666
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