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Kinetics of elementary steps in loop-mediated isothermal amplification (LAMP) show that strand invasion during initiation is rate-limiting

Loop-mediated isothermal amplification (LAMP) has proven to be easier to implement than PCR for point-of-care diagnostic tests. However, the underlying mechanism of LAMP is complicated and the kinetics of the major steps in LAMP have not been fully elucidated, which prevents rational improvements in...

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Detalles Bibliográficos
Autores principales: Dangerfield, Tyler L, Paik, Inyup, Bhadra, Sanchita, Johnson, Kenneth A, Ellington, Andrew D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841402/
https://www.ncbi.nlm.nih.gov/pubmed/36583345
http://dx.doi.org/10.1093/nar/gkac1221

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